Effect of Exhaustive Treatment of Human Erythrocyte White Gosts With PIPLC on the Activity of Acidic and Neutral Sialidases
| Enzyme Source and Treatment . | Acidic Sialidase ( μU/mg protein) . | Neutral Sialidase ( μU/mg protein) . |
|---|---|---|
| WG | 23.0 ± 0.9 | 1.2 ± 0.5 |
| PIPLC-treated | ||
| WG supernatant | 101.7 ± 4.7 | ND |
| WGP | ND | 52.8 ± 2.7 |
| Blank of PIPLC-treated | ||
| WG supernatant | ND | ND |
| WGP | 22.3 ± 1.1 | 53.2 ± 3.1 |
| WGP submitted to isoosmotic treatment | — | 63.9 ± 2.6 |
| Enzyme Source and Treatment . | Acidic Sialidase ( μU/mg protein) . | Neutral Sialidase ( μU/mg protein) . |
|---|---|---|
| WG | 23.0 ± 0.9 | 1.2 ± 0.5 |
| PIPLC-treated | ||
| WG supernatant | 101.7 ± 4.7 | ND |
| WGP | ND | 52.8 ± 2.7 |
| Blank of PIPLC-treated | ||
| WG supernatant | ND | ND |
| WGP | 22.3 ± 1.1 | 53.2 ± 3.1 |
| WGP submitted to isoosmotic treatment | — | 63.9 ± 2.6 |
PIPLC treatment was followed by centrifugation. The blank mixtures of PIPLC-treated white ghosts, in which PIPLC was not present, were also analyzed. The pellet obtained after white ghosts treatment with PIPLC was submitted to isoosmotic treatment, as reported in the legend to Table 1. For details, see Materials and Methods. The data are the mean values of four to five experiments ± SD values.
Abbreviations: WG, white ghosts; WGP, white ghost pellet; ND, not detectable.