Orientation of Sialidase in Human Erythrocyte Membranes
| Enzyme Source . | Sialidase ( μU/mg protein) . | Achesterase (U/mg protein) . | NADH-Cyt.C Ox/Red (mU/mg protein) . | |
|---|---|---|---|---|
| . | pH 4.7 . | pH 7.2 . | . | . |
| White ghosts | 23.0 ± 0.9 | 1.2 ± 0.05 | 2.86 ± 0.1 | 1.8 ± 0.04 |
| Resealed ghosts | 14.3 ± 0.7 | 56.9 ± 2.3 | 2.42 ± 0.1 | 1.8 ± 0.05 |
| Inside-out vesicles | ND | ND | 0.09 ± 0 | 20.0 ± 0.53 |
| Lysed inside-out vesicles | 16.4 ± 0.5 | 0.5 ± 0.02 | 1.95 ± 0.08 | 5.7 ± 0.23 |
| Enzyme Source . | Sialidase ( μU/mg protein) . | Achesterase (U/mg protein) . | NADH-Cyt.C Ox/Red (mU/mg protein) . | |
|---|---|---|---|---|
| . | pH 4.7 . | pH 7.2 . | . | . |
| White ghosts | 23.0 ± 0.9 | 1.2 ± 0.05 | 2.86 ± 0.1 | 1.8 ± 0.04 |
| Resealed ghosts | 14.3 ± 0.7 | 56.9 ± 2.3 | 2.42 ± 0.1 | 1.8 ± 0.05 |
| Inside-out vesicles | ND | ND | 0.09 ± 0 | 20.0 ± 0.53 |
| Lysed inside-out vesicles | 16.4 ± 0.5 | 0.5 ± 0.02 | 1.95 ± 0.08 | 5.7 ± 0.23 |
All enzyme activities are expressed as international units. For details, see Materials and Methods. The data are the mean values of four to five experiments ± SD values.
Abbreviation: ND, not detectable.