Primers Used in the PCR-SSCP Assay of CD95
| Primer . | Sequence . | Size of PCR Product (nt) . |
|---|---|---|
| 1-1F | 5′-GACAGGAATGCCCATTTGTG-3′ | 207 |
| 1-1FM | 5′-GACAGGAGTGCCCATTTGTG-3′ | |
| 1-1R | 5′-ACCTGTGTGTCACTCTTGCG-3′ | |
| 1-2F | 5′-GCTTTTCGTGAGCTCGTCTC-3′ | 206 |
| 1-2FM | 5′-GCTTTTCGTTAGCTCGTCTC-3′ | |
| 1-2FMA | 5′-GCTTTTCGTAAGCTCGTCTC-3′ | |
| 1-2R | 5′-CGTACTGAGCGGGTCCAC-3′ | |
| 1-3F | 5′-TGGACCCGCTCAGTACG-3′ | 135 |
| 1-3FM | 5′-TGGACCCGCACAGTACG-3′ | |
| 1-3R | 5′-GGACTAAGACGGGGTAAG-3′ | |
| 2-F | 5′-GTGGGTTACACTGGTTTACACGTTGC-3′ | 216 |
| 2-FM | 5′-GTGGGTTACACTCGTTTACACGTTGC-3′ | |
| 2-R | 5′-CATGATTACTATGTGCTACTCCTAACTG-3′ | |
| 3-F | 5′-TCCTGTTCAAACACTTGCTCC-3′ | 230 |
| 3-FM | 5′-TCCTGTTCAGACACTTGCTCC-3′ | |
| 3-R | 5′-GTTCTACATGAAATTCCAAGATTGG-3′ | |
| 3-RM | 5′-GTTCTACATGAAATTCTAAGATTGG-3′ | |
| 4-F | 5′-AGCCGCGATAACTAATAGTTTCC-3′ | 230 |
| 4-FM | 5′-AGCCGCGATAACTGATAGTTTCC-3′ | |
| 4-R | 5′-TCTCAGTCAGTGTTACTTCCCTAGG-3′ | |
| 4-RM | 5′-TCTCAGTCAGTGTAACTTCCCTAGG-3′ | |
| 5-F | 5′-TTGCCAGAGATGCAAAGATG-3′ | 213 |
| 5-FM | 5′-TTGCCAGAGCTGCAAAGATG-3′ | |
| 5-R | 5′-GGGGGAAAGGAGAATATAACC-3′ | |
| 6-F | 5′-TTCATATAAAATGTCCAATGTTCC-3′ | 123 |
| 6-FM | 5′-TTCATATAATATGTCCAATGTTCC-3′ | |
| 6-R | 5′-CTGCAGTTTGAACAAAGCAA-3′ | |
| 7-F | 5′-TCTCACATGCATTCTACAAGGC-3′ | 182 |
| 7-FM | 5′-TCTCACATGCAGTCTACAAGGC-3′ | |
| 7-FMA | 5′-TCTCACATGCACTCTACAAGGC-3′ | |
| 7-R | 5′-TTCTTTTCAAGGAAAGCTGATACC-3′ | |
| 8-F | 5′-TATTTTTATTTGTCTTTCTCTGCTTCC-3′ | 125 |
| 8-FM | 5′-TATTTTTATTTGTCTGTCTCTGCTTCC-3′ | |
| 8-FMA | 5′-TATTTTTATTTGTCTCTCTCTGCTTCC-3′ | |
| 8-R | 5′-TTGGCCTATTACTCTAAAGGATGC-3′ | |
| 9-1F | 5′-CATTTAGAAAAACAAATTTTCAGAC-3′ | 175 |
| 9-1FM | 5′-CATTTAGAAAAACAGATTTTCAGAC-3′ | |
| 9-1R | 5′-GGACATTGTCATTCTTGATCTC-3′ | |
| 9-2F | 5′-CAAGTTAAAGGCTTTGTTCG-3′ | 193 |
| 9-2FM | 5′-CAAGTTAATGGCTTTGTTCG-3′ | |
| 9-2R | 5′-CTGCAAGAGTACAAAGATTGG-3′ | |
| 9-3F | 5′-GCGTATGACACATTGATTAAAGATC-3′ | 168 |
| 9-3R | 5′-CAGAACTGAATTTGTTGTTTTTCAC-3′ | |
| 9-3RM | 5′-CAGAACTTGAATTTGCTGTTTTTCAC-3′ |
| Primer . | Sequence . | Size of PCR Product (nt) . |
|---|---|---|
| 1-1F | 5′-GACAGGAATGCCCATTTGTG-3′ | 207 |
| 1-1FM | 5′-GACAGGAGTGCCCATTTGTG-3′ | |
| 1-1R | 5′-ACCTGTGTGTCACTCTTGCG-3′ | |
| 1-2F | 5′-GCTTTTCGTGAGCTCGTCTC-3′ | 206 |
| 1-2FM | 5′-GCTTTTCGTTAGCTCGTCTC-3′ | |
| 1-2FMA | 5′-GCTTTTCGTAAGCTCGTCTC-3′ | |
| 1-2R | 5′-CGTACTGAGCGGGTCCAC-3′ | |
| 1-3F | 5′-TGGACCCGCTCAGTACG-3′ | 135 |
| 1-3FM | 5′-TGGACCCGCACAGTACG-3′ | |
| 1-3R | 5′-GGACTAAGACGGGGTAAG-3′ | |
| 2-F | 5′-GTGGGTTACACTGGTTTACACGTTGC-3′ | 216 |
| 2-FM | 5′-GTGGGTTACACTCGTTTACACGTTGC-3′ | |
| 2-R | 5′-CATGATTACTATGTGCTACTCCTAACTG-3′ | |
| 3-F | 5′-TCCTGTTCAAACACTTGCTCC-3′ | 230 |
| 3-FM | 5′-TCCTGTTCAGACACTTGCTCC-3′ | |
| 3-R | 5′-GTTCTACATGAAATTCCAAGATTGG-3′ | |
| 3-RM | 5′-GTTCTACATGAAATTCTAAGATTGG-3′ | |
| 4-F | 5′-AGCCGCGATAACTAATAGTTTCC-3′ | 230 |
| 4-FM | 5′-AGCCGCGATAACTGATAGTTTCC-3′ | |
| 4-R | 5′-TCTCAGTCAGTGTTACTTCCCTAGG-3′ | |
| 4-RM | 5′-TCTCAGTCAGTGTAACTTCCCTAGG-3′ | |
| 5-F | 5′-TTGCCAGAGATGCAAAGATG-3′ | 213 |
| 5-FM | 5′-TTGCCAGAGCTGCAAAGATG-3′ | |
| 5-R | 5′-GGGGGAAAGGAGAATATAACC-3′ | |
| 6-F | 5′-TTCATATAAAATGTCCAATGTTCC-3′ | 123 |
| 6-FM | 5′-TTCATATAATATGTCCAATGTTCC-3′ | |
| 6-R | 5′-CTGCAGTTTGAACAAAGCAA-3′ | |
| 7-F | 5′-TCTCACATGCATTCTACAAGGC-3′ | 182 |
| 7-FM | 5′-TCTCACATGCAGTCTACAAGGC-3′ | |
| 7-FMA | 5′-TCTCACATGCACTCTACAAGGC-3′ | |
| 7-R | 5′-TTCTTTTCAAGGAAAGCTGATACC-3′ | |
| 8-F | 5′-TATTTTTATTTGTCTTTCTCTGCTTCC-3′ | 125 |
| 8-FM | 5′-TATTTTTATTTGTCTGTCTCTGCTTCC-3′ | |
| 8-FMA | 5′-TATTTTTATTTGTCTCTCTCTGCTTCC-3′ | |
| 8-R | 5′-TTGGCCTATTACTCTAAAGGATGC-3′ | |
| 9-1F | 5′-CATTTAGAAAAACAAATTTTCAGAC-3′ | 175 |
| 9-1FM | 5′-CATTTAGAAAAACAGATTTTCAGAC-3′ | |
| 9-1R | 5′-GGACATTGTCATTCTTGATCTC-3′ | |
| 9-2F | 5′-CAAGTTAAAGGCTTTGTTCG-3′ | 193 |
| 9-2FM | 5′-CAAGTTAATGGCTTTGTTCG-3′ | |
| 9-2R | 5′-CTGCAAGAGTACAAAGATTGG-3′ | |
| 9-3F | 5′-GCGTATGACACATTGATTAAAGATC-3′ | 168 |
| 9-3R | 5′-CAGAACTGAATTTGTTGTTTTTCAC-3′ | |
| 9-3RM | 5′-CAGAACTTGAATTTGCTGTTTTTCAC-3′ |
The first numeral denotes the exon number (primers 1-1F to 1-3R include primers covering the promoter), a second numeral one of the overlapping primer pairs within an exon.
Abbreviations: F, forward primer; R, reverse primer; M, mutant primer; MA, additional mutant primer. Mutant bases are underlined.