LAK Activity of Cord Blood and BM Cells Against Daudi and YAC-1 Target Cells
. | Cord . | BM . | P Value . |
---|---|---|---|
Necrosis (Daudi) | 86.0 ± 11.6 (n = 8) | 45.7 ± 25.0 (n = 11) | <.001 |
Apoptosis (YAC-1) | 66.1 ± 9.7 (n = 5) | 2.2 ± 2.7 (n = 7) | <.001 |
. | Cord . | BM . | P Value . |
---|---|---|---|
Necrosis (Daudi) | 86.0 ± 11.6 (n = 8) | 45.7 ± 25.0 (n = 11) | <.001 |
Apoptosis (YAC-1) | 66.1 ± 9.7 (n = 5) | 2.2 ± 2.7 (n = 7) | <.001 |
Cord blood and BM mononuclear cells were isolated and cultured in IL-2 (500 U/mL). After 3 days, the cells were washed and their cytotoxicity against Daudi and YAC-1 cells was measured using the51Cr and 125I-UdR release assays, respectively. Figures show the % kill ± 1 SD. A constant E:T of 25:1 was used. The average spons/max ratios were 14.8% for the Daudi cell experiments and 10.0% for the YAC-1 cell experiments. The Mann Whitney statistical test was used to compare data.