Table 4. Comparison of In Vitro... | American Society of Hematology

Table 4.

Comparison of In Vitro Proliferative Response of Thymocytes and Splenic T Cells Between wt and hSCF ²²⁰ Mice

Genotype	Treatment	Thymocytes	Splenocytes
wt	PBS	0.33 ± 0.03	0.26 ± 0.006
	Anti-CD3 MoAb	0.78 ± 0.1^4-150	0.60 ± 0.06^4-150
hSCF ²²⁰	PBS	0.27 ± 0.02	0.36 ± 0.01
	Anti-CD3 MoAb	0.39 ± 0.04	0.76 ± 0.1^4-150

Genotype	Treatment	Thymocytes	Splenocytes
wt	PBS	0.33 ± 0.03	0.26 ± 0.006
	Anti-CD3 MoAb	0.78 ± 0.1^4-150	0.60 ± 0.06^4-150
hSCF ²²⁰	PBS	0.27 ± 0.02	0.36 ± 0.01
	Anti-CD3 MoAb	0.39 ± 0.04	0.76 ± 0.1^4-150

In vitro proliferation on thymocytes and splenic T cells from wt and hSCF ²²⁰ mice was performed as described in Materials and Methods. Thymoctyes and splenocytes were cultured in the presence of 10 μg/mL of anti-CD3 MoAb, and their proliferation monitored for 3 days. Proliferation was determined by measuring the optical density (OD) at 405 nm using an Elisa plate reader. At least three mice were examined per group. Data represent the mean ± SEM for each group from two separate experiments.

F4-150

P < .05 anti-CD3 v PBS control for wt thymocytes. *P < .01 anti-CD3 v PBS control for wt splenocytes and *P < .05 for hSCF ²²⁰ splenocytes. Anti-CD3 MoAb mediated proliferation of splenocytes from wt and hSCF ²²⁰ was not statistically different (P = .2).

View Large

This Feature Is Available To Subscribers Only