Effect of rmSCF on Proportions of CMCs Expressing MMCP-5, MMCP-6, or MC-CPA mRNA in +/+ or mi/mi CMCs Shown by In Situ Hybridization
| Genotype of CMCs . | Concentration of rmSCF . | Proportion of . | Proportion of . | Proportion of MC-CPA mRNA+ to Alcian Blue+ Cells (%)† . |
|---|---|---|---|---|
| . | (ng/mL)* . | MMCP-5 mRNA+ to Alcian Blue+ Cells (%)† . | MMCP-6 mRNA+ to Alcian Blue+ Cells (%)† . | . |
| +/+ | 0 | 45 ± 2 | 44 ± 3 | 92 ± 5 |
| mi/mi | 0 | 4 ± 3‡ | 3 ± 1‡ | 86 ± 4 |
| +/+ | 10 | 54 ± 7 | 52 ± 6 | 93 ± 3 |
| mi/mi | 10 | 41 ± 4ρ | 5 ± 4‡ | 85 ± 5 |
| +/+ | 50 | 52 ± 6 | 53 ± 7 | 86 ± 7 |
| mi/mi | 50 | 38 ± 4ρ | 5 ± 2‡ | 82 ± 6 |
| Genotype of CMCs . | Concentration of rmSCF . | Proportion of . | Proportion of . | Proportion of MC-CPA mRNA+ to Alcian Blue+ Cells (%)† . |
|---|---|---|---|---|
| . | (ng/mL)* . | MMCP-5 mRNA+ to Alcian Blue+ Cells (%)† . | MMCP-6 mRNA+ to Alcian Blue+ Cells (%)† . | . |
| +/+ | 0 | 45 ± 2 | 44 ± 3 | 92 ± 5 |
| mi/mi | 0 | 4 ± 3‡ | 3 ± 1‡ | 86 ± 4 |
| +/+ | 10 | 54 ± 7 | 52 ± 6 | 93 ± 3 |
| mi/mi | 10 | 41 ± 4ρ | 5 ± 4‡ | 85 ± 5 |
| +/+ | 50 | 52 ± 6 | 53 ± 7 | 86 ± 7 |
| mi/mi | 50 | 38 ± 4ρ | 5 ± 2‡ | 82 ± 6 |
+/+ or mi/mi CMCs were incubated with 10% PWM-SCM and various concentrations of rmSCF for 2 weeks.
Mean ± SE of five independent experiments.
P < .01 by t-test when compared with the value of +/+ CMCs, which were cultured without rmSCF.
ρ P < .01 by t-test when compared with the value of mi/mi CMCs, which were cultured without rmSCF.