Effect of Antibodies Against CD64, CD18, L-Selectin, and an RGD-Containing Peptide on the Binding of Sickle Cell PMNs to Endothelial Monolayers
| . | % Inhibition of Adhesion . | |||||||
|---|---|---|---|---|---|---|---|---|
| Anti-CD64 Antibodies . | Control Antibodies . | Anti-CD18 . | Anti–L-Selectin . | RGD Peptide . | ||||
| Clone 22 . | Clone 32.2 . | Clone 197 . | IgG1 . | IgG2 . | ||||
| Untreated endothelial monolayers | 42 ± 28 P = .053 (n = 6) | 33 ± 26* P = .01 (n = 7) | 21 ± 24* P = .03 (n = 14) | −7 ± 15 P ≥ .1 (n = 7) | −2 ± 9 P ≥ .1 (n = 14) | 45 ± 19* P > .1 (n = 6) | −4 ± 18 P > .1 (n = 6) | −2 ± 8 P ≥ .1 (n = 6) |
| TNF-treated endothelial monolayers | 36 ± 24 P = .052 (n = 6) | 26 ± 29 P = .068 (n = 7) | 29 ± 18* P = .004 (n = 14) | 3 ± 11 P ≥ .1 (n = 7) | −4 ± 13 P ≥ .1 (n = 14) | 43 ± 16* P > .1 (n = 6) | 19 ± 14 P > .1 (n = 6) | 6 ± 11 P ≥ .1 (n = 6) |
| . | % Inhibition of Adhesion . | |||||||
|---|---|---|---|---|---|---|---|---|
| Anti-CD64 Antibodies . | Control Antibodies . | Anti-CD18 . | Anti–L-Selectin . | RGD Peptide . | ||||
| Clone 22 . | Clone 32.2 . | Clone 197 . | IgG1 . | IgG2 . | ||||
| Untreated endothelial monolayers | 42 ± 28 P = .053 (n = 6) | 33 ± 26* P = .01 (n = 7) | 21 ± 24* P = .03 (n = 14) | −7 ± 15 P ≥ .1 (n = 7) | −2 ± 9 P ≥ .1 (n = 14) | 45 ± 19* P > .1 (n = 6) | −4 ± 18 P > .1 (n = 6) | −2 ± 8 P ≥ .1 (n = 6) |
| TNF-treated endothelial monolayers | 36 ± 24 P = .052 (n = 6) | 26 ± 29 P = .068 (n = 7) | 29 ± 18* P = .004 (n = 14) | 3 ± 11 P ≥ .1 (n = 7) | −4 ± 13 P ≥ .1 (n = 14) | 43 ± 16* P > .1 (n = 6) | 19 ± 14 P > .1 (n = 6) | 6 ± 11 P ≥ .1 (n = 6) |
Results are expressed as the mean percentage ± SD inhibition of PMN adherence to endothelium. Isotype control antibodies were IgG1 for clones 22 and 32.2 and IgG2a for clone 197. Adherence of PMNs to untreated endothelium was inhibited by two of the anti-CD64 antibodies (clones 32.2 and 197) and by the anti-CD18 antibody. Binding to TNF-treated endothelium was inhibited by antibodies of clone 197 and anti-CD18.
Abbreviation: n, number of patients studied.
P < .05 was considered to be a significant inhibition of adhesion.