Enriched CD64+ PMNs Are Highly Adherent to Endothelial Monolayers
| Experiment No. . | No. of Adherent Cells/High Power Field . | |
|---|---|---|
| CD64+ . | CD64− . | |
| 1 | 89 ± 25 | 6 ± 4 |
| 2 | 33 ± 11 | 12 ± 3 |
| 3 | 56 ± 6 | 6 ± 1 |
| 4 | 65 ± 10 | 8 ± 4 |
| Mean ± SD | 61 ± 23* | 8 ± 3 |
| Experiment No. . | No. of Adherent Cells/High Power Field . | |
|---|---|---|
| CD64+ . | CD64− . | |
| 1 | 89 ± 25 | 6 ± 4 |
| 2 | 33 ± 11 | 12 ± 3 |
| 3 | 56 ± 6 | 6 ± 1 |
| 4 | 65 ± 10 | 8 ± 4 |
| Mean ± SD | 61 ± 23* | 8 ± 3 |
PMNs from healthy control subjects were separated into CD64+ and CD64− cells on a FACSorter (see Materials and Methods), and both populations were applied to untreated endothelial monolayers. Data are expressed as the mean ± SD number of adherent cells counted per high power field in triplicate assays. Results show that CD64+ PMNs are far more adherent than CD64− PMNs to endothelial monolayers prepared from the same umbilical cord vein.
P = .004 by the unpaired Student's t-test.