Effect of OA on Platelet Adhesion to Collagen Mediated by the α2β1 Integrin
| Experimental Situation . | Extent of Adhesion (% singlets bound) . | Inhibition (%) . |
|---|---|---|
| Control | 51.0 ± 2.9 | 0 |
| OA (0.1 μmol/L) | 49.7 ± 2.9 | 2.6 |
| OA (1.0 μmol/L) | 20.3 ± 2.0 | 60.2 |
| Experimental Situation . | Extent of Adhesion (% singlets bound) . | Inhibition (%) . |
|---|---|---|
| Control | 51.0 ± 2.9 | 0 |
| OA (0.1 μmol/L) | 49.7 ± 2.9 | 2.6 |
| OA (1.0 μmol/L) | 20.3 ± 2.0 | 60.2 |
Washed platelets were pumped through a micro-adhesion column containing collagen-coated Sepharose beads at a shear rate of 1,700 s−1, giving an adhesion or contact time of 1.37 seconds, as described in the Materials and Methods. Where indicated, the platelets were pretreated with either 0.1 or 1 μmol/L OA dissolved in 0.1% (vol/vol) DMSO for 10 minutes at 37°C. Control platelets were incubated with 0.1% (vol/vol) DMSO. Adhesion data are means ± SD from three separate platelet preparations, with nonspecific adhesion of 7% having been subtracted from these values.26 The inhibition data reflect the percentage of effect of OA compared with the control adhesion value.