Quantification of Changes in Ceramide, Sphingomyelin, and Gb3/CD77 Levels in Ramos Cells Treated by 38.13 Anti-CD77 MoAb
| . | Incubation Times (min) . | |||||
|---|---|---|---|---|---|---|
| . | 0 . | 15 . | 30 . | 60 . | 90 . | 120 . |
| Ceramide | 1 | 1.03 ± 0.05 | 1.10 ± 0.08 | 1.35 ± 0.12 | 1.77 ± 0.22 | 1.09 ± 0.10 |
| Sphingomyelin | 1 | 1.12 ± 0.07 | 1.00 ± 0.04 | 1.11 ± 0.09 | 1.29 ± 0.19 | 1.12 ± 0.10 |
| CD77 | 1 | 0.97 ± 0.07 | 1.10 ± 0.16 | 1.00 ± 0.12 | 1.17 ± 0.08 | 0.92 ± 0.04 |
| . | Incubation Times (min) . | |||||
|---|---|---|---|---|---|---|
| . | 0 . | 15 . | 30 . | 60 . | 90 . | 120 . |
| Ceramide | 1 | 1.03 ± 0.05 | 1.10 ± 0.08 | 1.35 ± 0.12 | 1.77 ± 0.22 | 1.09 ± 0.10 |
| Sphingomyelin | 1 | 1.12 ± 0.07 | 1.00 ± 0.04 | 1.11 ± 0.09 | 1.29 ± 0.19 | 1.12 ± 0.10 |
| CD77 | 1 | 0.97 ± 0.07 | 1.10 ± 0.16 | 1.00 ± 0.12 | 1.17 ± 0.08 | 0.92 ± 0.04 |
For ceramide, each value is: pmol in 38.13 treated cells ÷ pmol in control cells. For sphingomyelin and Gb3/CD77, each spot of the HPTLC obtained from cells treated or not with 38.13 MoAb was scraped off the plates and quantitated by liquid scintillation counting. Each value is: cpm in 38.13 treated cells ÷ cpm in control cells. Results given are the means ± SD of five independent experiments.