Primers Used in Reverse Transcription, PCR, and Sequencing
| Primer . | Sequence (5′ → 3′) . | Nucleotides Amplified* . |
|---|---|---|
| 1 (5′) | GCACCGGCTACAACGGGATCT | 1281-1301 |
| 2 (3′) | TCCCCGAATCCTACAGGGAAC | 1600-1620 |
| 3 (5′) | CTCACCACTGCTGTGCTCCTG | 1382-1402 |
| 4 (5′) | CTGCGCAGAAAGTGGGAGGAG | 1436-1456 |
| 5 (3′) | TGGAACAGGTGGCTCTTGCTG | 1471-1491 |
| 6 (3′) | ACTCCCGCTAGTGAAGGCCGA | 1529-1549 |
| 7 (3′) | CAGGAGCACAGCAGTGGTGAG | 1382-1402 |
| 8 (5′) | CAGAACGGGAGCGCAGAGCTT | 1490-1510 |
| 9 (3′) | CAGGTAGGGCCCATTGAAGTC | 1790-1810 |
| 10 (5′) | CCAGAGCTGACCAGGGAGAT | 11-30 |
| Primer . | Sequence (5′ → 3′) . | Nucleotides Amplified* . |
|---|---|---|
| 1 (5′) | GCACCGGCTACAACGGGATCT | 1281-1301 |
| 2 (3′) | TCCCCGAATCCTACAGGGAAC | 1600-1620 |
| 3 (5′) | CTCACCACTGCTGTGCTCCTG | 1382-1402 |
| 4 (5′) | CTGCGCAGAAAGTGGGAGGAG | 1436-1456 |
| 5 (3′) | TGGAACAGGTGGCTCTTGCTG | 1471-1491 |
| 6 (3′) | ACTCCCGCTAGTGAAGGCCGA | 1529-1549 |
| 7 (3′) | CAGGAGCACAGCAGTGGTGAG | 1382-1402 |
| 8 (5′) | CAGAACGGGAGCGCAGAGCTT | 1490-1510 |
| 9 (3′) | CAGGTAGGGCCCATTGAAGTC | 1790-1810 |
| 10 (5′) | CCAGAGCTGACCAGGGAGAT | 11-30 |
*Nucleotides are numbered according to published GMRβ sequence.17