The Initial Velocity vi (% Analogues/Min) and the Steady-State Distribution (% of PE Analogues on the Cytoplasmic Leaflet) of SL-PE Redistribution at Different pH at 4°C
| Extracellular pH . | pH 5.8 . | pH 7.4 . | pH 8.5 . |
|---|---|---|---|
| vi (% analogues/min) | 0.22 ± 0.03 (4)* | 0.57 ± 0.06 (4) | 0.79 ± 0.2 (2)† |
| Plateau (% of PE inside) | 57.8 ± 5.1* | 87.0 ± 1.8 | 84.6 ± 5.8† |
| Extracellular pH . | pH 5.8 . | pH 7.4 . | pH 8.5 . |
|---|---|---|---|
| vi (% analogues/min) | 0.22 ± 0.03 (4)* | 0.57 ± 0.06 (4) | 0.79 ± 0.2 (2)† |
| Plateau (% of PE inside) | 57.8 ± 5.1* | 87.0 ± 1.8 | 84.6 ± 5.8† |
Data were obtained by nonlinear regression of redistribution kinetics to a monoexponential function. The average ± standard error of estimate of n measurements (in parentheses) is given. Statistical significance between data was tested by the t-test for unpaired observations at a significance level of α = .05.
Significant with respect to values at pH 7.4.
Not significant with respect to values at pH 7.4.