E Protein Expression During Erythroid Differentiation in Normal Donors and DBA
| Transcription . | Quantity (OD × mm) . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Factor . | . | Day 7 . | Day 10 . | Day 14 . | n . | . | |||
| . | . | SCF − . | + . | − . | + . | − . | + . | . | . |
| A. Normal donor | 3.26 ± 0.78 | 6.07 ± 0.893-150 | 3.14 ± 0.52 | 4.66 ± 0.873-150 | 1.15 ± 0.36 | 0.47 ± 0.14 | 11 | ||
| E47 | |||||||||
| HEB | 1.38 ± 0.23 | 2.75 ± 0.373-150 | 1.65 ± 0.46 | 3.22 ± 1.073-150 | 0.78 ± 0.36 | 0.53 ± 0.17 | 7 | ||
| B. Diamond-Blackfan | — | — | 0.77 ± 0.213-151 | 1.94 ± 0.54*† | 0.27 ± 0.093-151 | 0.51 ± 0.15 | 6 | ||
| E47 | |||||||||
| HEB | — | — | 0.50 ± 0.213-151 | 1.31 ± 0.243-150 | 0.31 ± 0.11 | 0.23 ± 0.16 | 6 | ||
| Transcription . | Quantity (OD × mm) . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Factor . | . | Day 7 . | Day 10 . | Day 14 . | n . | . | |||
| . | . | SCF − . | + . | − . | + . | − . | + . | . | . |
| A. Normal donor | 3.26 ± 0.78 | 6.07 ± 0.893-150 | 3.14 ± 0.52 | 4.66 ± 0.873-150 | 1.15 ± 0.36 | 0.47 ± 0.14 | 11 | ||
| E47 | |||||||||
| HEB | 1.38 ± 0.23 | 2.75 ± 0.373-150 | 1.65 ± 0.46 | 3.22 ± 1.073-150 | 0.78 ± 0.36 | 0.53 ± 0.17 | 7 | ||
| B. Diamond-Blackfan | — | — | 0.77 ± 0.213-151 | 1.94 ± 0.54*† | 0.27 ± 0.093-151 | 0.51 ± 0.15 | 6 | ||
| E47 | |||||||||
| HEB | — | — | 0.50 ± 0.213-151 | 1.31 ± 0.243-150 | 0.31 ± 0.11 | 0.23 ± 0.16 | 6 | ||
PB BFU-E from normal donors and patients with DBA were cultured with or without 100 ng/mL human SCF and removed from culture on day 7, 10, or 14. The cell lysate from 2 × 105 BFU-E derived cells was loaded onto each lane and immunoblotting performed with anti-E47 or anti-HEB antibodies. The densities of bands were measured and the mean ± SEM is shown. n = number of individuals studied.
Significantly increased E protein expression in response to SCF (P < .05).
Significantly decreased E protein expression compared to normal donors (P < .05).