Induction of T-Cell Stimulation by Bone Marrow CD38++ Plasma Cells, CD38+ Cells, and Antigen-Presenting Cells
| APC . | MLR (cpm) . | Ag-Induced Cell Proliferation (cpm) . | No. of Ag-Induced IFN-γ–Secreting Cells . | |||||
|---|---|---|---|---|---|---|---|---|
| . | APC Only . | APC + AlloPBMCs . | No Ag . | PPD . | TT . | No Ag . | PPD . | TT . |
| CD38++ | 462 (304-786) | 14,753 (4,370-35,670) | 396 (313-554) | 1,770 (944-2,391) | 1,524 (1,314-2,140) | 5 (4-8) | 39 (26-40) | 33 (22-39) |
| CD38+ | 459 (242-870) | 10,451 (7,511-34,307) | 477 (367-898) | 1,527 (917-2,739) | 2,075 (2,007-2,122) | 6 (4-12) | 33 (17-43) | 25 (15-35) |
| BMMCs | 542 (260-623) | 11,600 (6,344-40,151) | 504 (359-980) | 1,570 (596-3,573) | 1,893 (688-2,391) | 5 (4-9) | 30 (25-37) | 22 (18-30) |
| PBMCs | 482 (170-526) | 11,989 (7,001-31,474) | ND | ND | ND | ND | ND | ND |
| Mø | ND | ND | 418 (376-772) | 1,455 (697-1,883) | 1,058 (1,010-1,485) | 6 (3-7) | 28 (17-43) | 27 (27-33) |
| No APCs | 422 (302-1,373)3-150 | 431 (275-732)3-151 | 537 (283-950)3-151 | 659 (560-680)3-151 | 5 (3-12)3-151 | 6 (4-14)3-151 | 7 (5-12)3-151 | |
| APC . | MLR (cpm) . | Ag-Induced Cell Proliferation (cpm) . | No. of Ag-Induced IFN-γ–Secreting Cells . | |||||
|---|---|---|---|---|---|---|---|---|
| . | APC Only . | APC + AlloPBMCs . | No Ag . | PPD . | TT . | No Ag . | PPD . | TT . |
| CD38++ | 462 (304-786) | 14,753 (4,370-35,670) | 396 (313-554) | 1,770 (944-2,391) | 1,524 (1,314-2,140) | 5 (4-8) | 39 (26-40) | 33 (22-39) |
| CD38+ | 459 (242-870) | 10,451 (7,511-34,307) | 477 (367-898) | 1,527 (917-2,739) | 2,075 (2,007-2,122) | 6 (4-12) | 33 (17-43) | 25 (15-35) |
| BMMCs | 542 (260-623) | 11,600 (6,344-40,151) | 504 (359-980) | 1,570 (596-3,573) | 1,893 (688-2,391) | 5 (4-9) | 30 (25-37) | 22 (18-30) |
| PBMCs | 482 (170-526) | 11,989 (7,001-31,474) | ND | ND | ND | ND | ND | ND |
| Mø | ND | ND | 418 (376-772) | 1,455 (697-1,883) | 1,058 (1,010-1,485) | 6 (3-7) | 28 (17-43) | 27 (27-33) |
| No APCs | 422 (302-1,373)3-150 | 431 (275-732)3-151 | 537 (283-950)3-151 | 659 (560-680)3-151 | 5 (3-12)3-151 | 6 (4-14)3-151 | 7 (5-12)3-151 | |
Median and range (in parentheses) are shown. The results were obtained from experiments using an optimal amount of antigen-presenting cells from five MM patients.
Abbreviations: Ag, antigen; APCs, antigen-presenting cells; AlloPBMCs, allogeneic PBMCs; ND, not done; Mø, monocytes.
Data obtained from cultures with allogeneic PBMCs only.
Data obtained from cultures with purified T cells only.