Factor-Dependent and -Independent Colonies Formed by Fetal Liver Cells Infected With Wild-Type and Mutant hβc Subunits
| RufNeo Construct . | Additions . | Colony types (%) . | Colonies Examined . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | G . | Eo . | Mast . | M . | GM . | Mix . | BFU-E . | Blast . | . |
| hβc | IL-3, GM, Epo | 12 | 1 | 4 | 39 | 33 | 8 | 4 | — | 103 |
| IL-3, GM, Epo, G418 | 18 | 3 | 6 | 38 | 26 | 5 | 5 | — | 109 | |
| FIΔ | Epo | 34 | — | — | 24 | 36 | — | 6 | — | 80 |
| — | 17 | — | — | 45 | 38 | — | — | — | 84 | |
| V449E | Epo | 9 | 1 | 12 | 26 | 17 | 29 | 3 | 3 | 69 |
| — | 13 | 8 | 7 | 30 | 20 | 18 | — | 5 | 61 | |
| I374N | Epo | 4 | — | — | 79 | 15 | — | 1 | — | 73 |
| — | 1 | — | — | 89 | 10 | — | — | — | 93 | |
| RufNeo Construct . | Additions . | Colony types (%) . | Colonies Examined . | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| . | . | G . | Eo . | Mast . | M . | GM . | Mix . | BFU-E . | Blast . | . |
| hβc | IL-3, GM, Epo | 12 | 1 | 4 | 39 | 33 | 8 | 4 | — | 103 |
| IL-3, GM, Epo, G418 | 18 | 3 | 6 | 38 | 26 | 5 | 5 | — | 109 | |
| FIΔ | Epo | 34 | — | — | 24 | 36 | — | 6 | — | 80 |
| — | 17 | — | — | 45 | 38 | — | — | — | 84 | |
| V449E | Epo | 9 | 1 | 12 | 26 | 17 | 29 | 3 | 3 | 69 |
| — | 13 | 8 | 7 | 30 | 20 | 18 | — | 5 | 61 | |
| I374N | Epo | 4 | — | — | 79 | 15 | — | 1 | — | 73 |
| — | 1 | — | — | 89 | 10 | — | — | — | 93 | |
Data are a combination of two separate experiments. Colony types were determined at day 7 of culture.
Abbreviations: G, neutrophilic granulocyte; Eo, eosinophil; Mast, mast cell; M, macrophage; GM, granulocyte-macrophage; Mix, mixed myeloid/erythroid; BFU-E, erythroid burst-forming unit; Blast, blast cell; GM, GM-CSF.