Differential Cell Counts of Factor-Dependent and Factor-Independent Fetal Liver Cells
| RufNeo Construct . | Additions . | Cell Type (%) . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Eos . | Mast . | Neut . | MK . | M . | Eryth . | Myel . | Blast . |
| Day 7 | |||||||||
| hβc | IL-3, GM, Epo | 0.6 | 2.9 | 32.8 | 0.6 | 27.0 | 4.6 | 26.4 | 5.2 |
| IL3, GM, Epo, G418 | 1.0 | — | 45.5 | 0.3 | 18.3 | 9.3 | 20.6 | 5.0 | |
| FIΔ | Epo | — | — | 67.3 | — | 28.7 | 2.7 | 1.3 | — |
| — | — | — | 69.7 | — | 30.3 | — | — | — | |
| V449E | Epo | 0.3 | 1.3 | 47.8 | 0.3 | 4.3 | 10.0 | 32.6 | 3.3 |
| — | 2.0 | 0.7 | 48.8 | 0.7 | 0.7 | 2.3 | 39.9 | 5.0 | |
| I374N | Epo | — | — | 4.0 | — | 92.9 | 3.2 | — | — |
| — | — | — | 11.2 | — | 88.8 | — | — | — | |
| Day 21 | |||||||||
| hβc | IL-3, GM, Epo | — | 9.0 | — | — | 41.0 | 41.0 | 9.0 | — |
| IL-3, GM, Epo, G418 | — | 1.0 | 0.3 | — | 79.3 | 13.3 | 6.0 | — | |
| FIΔ | Epo | — | — | — | — | 99.3 | 0.7 | — | — |
| — | — | — | — | — | 100.0 | — | — | — | |
| V449E | Epo | — | 8.3 | 6.0 | — | — | 1.3 | 72.0 | 12.3 |
| — | — | 0.7 | 2.7 | — | — | — | 93.3 | 3.3 | |
| I374N | Epo | — | — | — | — | 100.0 | — | — | — |
| — | — | — | — | — | 100.0 | — | — | — | |
| RufNeo Construct . | Additions . | Cell Type (%) . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| . | . | Eos . | Mast . | Neut . | MK . | M . | Eryth . | Myel . | Blast . |
| Day 7 | |||||||||
| hβc | IL-3, GM, Epo | 0.6 | 2.9 | 32.8 | 0.6 | 27.0 | 4.6 | 26.4 | 5.2 |
| IL3, GM, Epo, G418 | 1.0 | — | 45.5 | 0.3 | 18.3 | 9.3 | 20.6 | 5.0 | |
| FIΔ | Epo | — | — | 67.3 | — | 28.7 | 2.7 | 1.3 | — |
| — | — | — | 69.7 | — | 30.3 | — | — | — | |
| V449E | Epo | 0.3 | 1.3 | 47.8 | 0.3 | 4.3 | 10.0 | 32.6 | 3.3 |
| — | 2.0 | 0.7 | 48.8 | 0.7 | 0.7 | 2.3 | 39.9 | 5.0 | |
| I374N | Epo | — | — | 4.0 | — | 92.9 | 3.2 | — | — |
| — | — | — | 11.2 | — | 88.8 | — | — | — | |
| Day 21 | |||||||||
| hβc | IL-3, GM, Epo | — | 9.0 | — | — | 41.0 | 41.0 | 9.0 | — |
| IL-3, GM, Epo, G418 | — | 1.0 | 0.3 | — | 79.3 | 13.3 | 6.0 | — | |
| FIΔ | Epo | — | — | — | — | 99.3 | 0.7 | — | — |
| — | — | — | — | — | 100.0 | — | — | — | |
| V449E | Epo | — | 8.3 | 6.0 | — | — | 1.3 | 72.0 | 12.3 |
| — | — | 0.7 | 2.7 | — | — | — | 93.3 | 3.3 | |
| I374N | Epo | — | — | — | — | 100.0 | — | — | — |
| — | — | — | — | — | 100.0 | — | — | — | |
Data are representative of three separate experiments.
Abbreviations: Eos, eosinophils; Mast, mast cells; Neut, neutrophils; MK, megakaryocytes; M, monocytes/macrophages; Eryth, erythroid cells; Myel, promyelocytes/myelocytes; Blast, blast cells; GM, GM-CSF.