Maximal Response to Epo for Transgenic and Control Adult Bone Marrow Erythroid Progenitors
| CFU-E–derived colonies | |||||||||
| Epo U/mL | 1 | 0.25 | 0.1 | 0.05 | 0.025 | 0.01 | 0.005 | 0.001 | 0.0005 |
| Transgenic | 100 | 94 ± 28 | 74 ± 15 | 42 ± 11 | 41 ± 5 | 35 ± 8 | 17 ± 5 | 18 ± 6 | 12 ± 0.1 |
| Control | 100 | 50 ± 14 | 44 ± 21 | 16 ± 6 | 17 ± 6 | 17 ± 4 | 4 ± 1 | 7 ± 2 | 1 ± 0.1 |
| P | — | <.01 | <.02 | <.001 | <.001 | <.001 | <.001 | <.001 | <.001 |
| BFU-E–derived colonies | |||||||||
| Epo U/mL | 4 | 1 | 0.4 | 0.1 | 0.025 | ||||
| Transgenic | 100 | 60 ± 10 | 31 ± 7 | 19 ± 7 | 3 ± 3 | ||||
| Control | 100 | 48 ± 22 | 18 ± 9 | 3 ± 1 | 0 | ||||
| P | — | NS | NS | <.05 | NS |
| CFU-E–derived colonies | |||||||||
| Epo U/mL | 1 | 0.25 | 0.1 | 0.05 | 0.025 | 0.01 | 0.005 | 0.001 | 0.0005 |
| Transgenic | 100 | 94 ± 28 | 74 ± 15 | 42 ± 11 | 41 ± 5 | 35 ± 8 | 17 ± 5 | 18 ± 6 | 12 ± 0.1 |
| Control | 100 | 50 ± 14 | 44 ± 21 | 16 ± 6 | 17 ± 6 | 17 ± 4 | 4 ± 1 | 7 ± 2 | 1 ± 0.1 |
| P | — | <.01 | <.02 | <.001 | <.001 | <.001 | <.001 | <.001 | <.001 |
| BFU-E–derived colonies | |||||||||
| Epo U/mL | 4 | 1 | 0.4 | 0.1 | 0.025 | ||||
| Transgenic | 100 | 60 ± 10 | 31 ± 7 | 19 ± 7 | 3 ± 3 | ||||
| Control | 100 | 48 ± 22 | 18 ± 9 | 3 ± 1 | 0 | ||||
| P | — | NS | NS | <.05 | NS |
Results from Fig 6 are expressed as percentages of the maximal response, defined at 1 U/mL and 4 U/mL Epo for CFU-E and BFU-E, respectively. Data were obtained from duplicate cultures in seven (CFU-E) and three (BFU-E) independent experiments.
Abbreviation: NS, not significant.