Acute LCMV Infection Results in Virus-Specific CTL Memory in the Spleen and Bone Marrow
| Days Postinfection . | LU/106 Total Cells (range)* . | LU/106 CD8+ T Cells (range)* . | ||
|---|---|---|---|---|
| . | Spleen . | Bone Marrow . | Spleen . | Bone Marrow . |
| 85 | 140 (108-171) | 10 (7-14) | 873 (675-1,070) | 690 (450-930) |
| 230 | 117 (92-146) | 17 (12-23) | 1,314 (1,028-1,635) | 1,223 (888-1,731) |
| 325 | 173 (141-200) | 23 (19-29) | 1,131 (975-1,247) | 1,166 (914-1,392) |
| CTLp Frequency/Total Cells† | CTLp Frequency/CD8+ T Cells† | |||
| Early | ||||
| 8 | ||||
| 1 | 1/4.7 × 103 | 1/4.3 × 104 | 1/6.6 × 102 | 1/6.5 × 102 |
| 2 | 1/9.3 × 102 | 1/1.5 × 104 | 1/1.4 × 102 | 1/2.1 × 102 |
| 3 | 1/1.6 × 103 | 1/1.4 × 104 | 1/2.2 × 102 | 1/2.0 × 102 |
| Late | ||||
| 57‡ | 1/1.8 × 104 | 1/1.3 × 105 | 1/2.8 × 103 | 1/2.0 × 103 |
| 230 | ||||
| 1 | 1/2.1 × 104 | 1/2.2 × 105 | 1/1.9 × 103 | 1/2.9 × 103 |
| 2 | 1/2.1 × 104 | 1/2.1 × 105 | 1/1.9 × 103 | 1/2.7 × 103 |
| Days Postinfection . | LU/106 Total Cells (range)* . | LU/106 CD8+ T Cells (range)* . | ||
|---|---|---|---|---|
| . | Spleen . | Bone Marrow . | Spleen . | Bone Marrow . |
| 85 | 140 (108-171) | 10 (7-14) | 873 (675-1,070) | 690 (450-930) |
| 230 | 117 (92-146) | 17 (12-23) | 1,314 (1,028-1,635) | 1,223 (888-1,731) |
| 325 | 173 (141-200) | 23 (19-29) | 1,131 (975-1,247) | 1,166 (914-1,392) |
| CTLp Frequency/Total Cells† | CTLp Frequency/CD8+ T Cells† | |||
| Early | ||||
| 8 | ||||
| 1 | 1/4.7 × 103 | 1/4.3 × 104 | 1/6.6 × 102 | 1/6.5 × 102 |
| 2 | 1/9.3 × 102 | 1/1.5 × 104 | 1/1.4 × 102 | 1/2.1 × 102 |
| 3 | 1/1.6 × 103 | 1/1.4 × 104 | 1/2.2 × 102 | 1/2.0 × 102 |
| Late | ||||
| 57‡ | 1/1.8 × 104 | 1/1.3 × 105 | 1/2.8 × 103 | 1/2.0 × 103 |
| 230 | ||||
| 1 | 1/2.1 × 104 | 1/2.2 × 105 | 1/1.9 × 103 | 1/2.9 × 103 |
| 2 | 1/2.1 × 104 | 1/2.1 × 105 | 1/1.9 × 103 | 1/2.7 × 103 |
Adult BALB/c mice were infected intraperitoneally with 2 × 105 PFU LCMV-Armstrong, and virus-specific CTL activity was determined after in vitro restimulation with virus for 5 days at the indicated times postinfection.
Defined as the number of effector cells required to exhibit 20% lysis of infected targets. The percentage of CD8+ T cells in the spleen and bone marrow was calculated by FACS prior to in vitro stimulation, and the range is given for 3 to 4 mice per group.
Determined by limiting dilution analysis as previously described.14
CTLp frequency at 57 days postinfection was calculated from a pool of 20 LCMV-immune mice.