Primary LCMV-Specific Effector CTL Response at 8 Days Postinfection
| Cell Sample . | Ex Vivo Virus-Specific CTLs (% specific 51Cr release)* . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Infected Targets . | Uninfected Targets . | LU† . | . | . | . | . | |||||||
| . | 100:1 . | 30:1 . | 10:1 . | 3:1 . | 100:1 . | 30:1 . | 10:1 . | Per 106 Cells . | Per 106 CD8+ Cells . | Per Organ or in Circulation . | . | . | . | . |
| Spleen | ND | 82 ± 1 | 50 ± 6 | 19 ± 2 | ND | 3 ± 1 | 0 | 24 ± 8 | 96 ± 30 | 2,928 ± 612 | ||||
| PBMCs‡ | 72 | 69 | 53 | 30 | 0 | 0 | 0 | 25 | 72 | 579 | ||||
| Bone marrow | 19 ± 1 | 8 ± 1 | 3 ± 1 | 1 ± 1 | 0 | 0 | 0 | 0.71 ± 0.08 | 36 ± 4 | 162 ± 27 | ||||
| Cell Sample . | Ex Vivo Virus-Specific CTLs (% specific 51Cr release)* . | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | Infected Targets . | Uninfected Targets . | LU† . | . | . | . | . | |||||||
| . | 100:1 . | 30:1 . | 10:1 . | 3:1 . | 100:1 . | 30:1 . | 10:1 . | Per 106 Cells . | Per 106 CD8+ Cells . | Per Organ or in Circulation . | . | . | . | . |
| Spleen | ND | 82 ± 1 | 50 ± 6 | 19 ± 2 | ND | 3 ± 1 | 0 | 24 ± 8 | 96 ± 30 | 2,928 ± 612 | ||||
| PBMCs‡ | 72 | 69 | 53 | 30 | 0 | 0 | 0 | 25 | 72 | 579 | ||||
| Bone marrow | 19 ± 1 | 8 ± 1 | 3 ± 1 | 1 ± 1 | 0 | 0 | 0 | 0.71 ± 0.08 | 36 ± 4 | 162 ± 27 | ||||
Adult BALB/c mice were infected intraperitoneally with 2 × 105 PFU LCMV-Armstrong, and cytolytic virus-specific CTL activity was determined in the spleen, peripheral blood, and bone marrow at 8 days postinfection.
Abbreviation: ND, not determined.
Without any in vitro stimulation, antiviral CTL activity exhibited by spleen, PBMCs, and bone marrow cells was determined by measuring specific 51Cr release from either uninfected or LCMV-infected targets at the indicated effector:target (E:T) ratios.
Lytic units (LU) ar defined as the number of effector cells required to exhibit 20% lysis of infected targets. The spleen and PBMCs contained approximately 30% CD8+ T cells, and CD8+ T cells accounted for about 2.0% of total cells in the bone marrow. LU per total organ were calculated based on total cells recovered from spleen, were estimated for bone marrow from 2 femurs (∼12% of total bone marrow27 ), and were estimated for PBMCs assuming 2.5 mL circulating blood per adult mouse.
Data pooled from 3 mice.