Effect of C-C and CXC Chemokines on HIV-1 Replication When Present Throughout Phases I + II
| Chemokine . | p24 Antigen Detection (ng/mL) (days postinfection) . | |||
|---|---|---|---|---|
| . | 3 . | 6 . | 9 . | 12 . |
| Eotaxin | 0.089 | 0.099 | 5.79 | 18 |
| MIP-1α | 0.088 | 0.274 | 0.347 | 0.401 |
| MIP-1β | 0.097 | 0.211 | 0.327 | 0.597 |
| MCP-1 | 0.113 | 1.904 | 7.859 | 29.13 |
| MCP-4 | 0.092 | 2.611 | 18.7 | 39.8 |
| IP10 | 0.308 | 33.4 | 40 | 154.6 |
| None | 0.18 | 4.045 | 25.1 | 76.6 |
| Chemokine . | p24 Antigen Detection (ng/mL) (days postinfection) . | |||
|---|---|---|---|---|
| . | 3 . | 6 . | 9 . | 12 . |
| Eotaxin | 0.089 | 0.099 | 5.79 | 18 |
| MIP-1α | 0.088 | 0.274 | 0.347 | 0.401 |
| MIP-1β | 0.097 | 0.211 | 0.327 | 0.597 |
| MCP-1 | 0.113 | 1.904 | 7.859 | 29.13 |
| MCP-4 | 0.092 | 2.611 | 18.7 | 39.8 |
| IP10 | 0.308 | 33.4 | 40 | 154.6 |
| None | 0.18 | 4.045 | 25.1 | 76.6 |
Purified DC (1 × 105 cells) from a single donor were preincubated with chemokines (250 ng/mL) for 60 minutes. The cells were then pulsed with HIV-1 (Yu-2, M-tropic virus; 3 ng/mL of p24 antigen) for 60 minutes. The cells were washed five times and cocultivated with activated, autologous T cells and chemokines.