Table 3. Sequences of Dominant TCRγ... | American Society of Hematology

Table 3.

Sequences of Dominant TCRγ Rearrangements

Diagnosis	Patient No.	Site	Sequencing	V-Segment	N-Region^3-150	J-Segment

MF IA	Ma	Sk	D + C	V8-ATTACTGTGCCACCTGGGAT	GAGC	AATTATTATAAGAAACTCTTTGG-J1/2
MF IA	Ma	Bl	D + C	V8-ATTACTGTGCCACCTGGGAT	GAGC	AATTATTATAAGAAACTCTTTGG-J1/2
MF IB	Ha	Sk	D + C	V2-ATTACTGTGCCACCTGGGACG	TACCTCGCCGGTAGG	AAGAAACTCTTTGG-J1/2
MF IB	Ha	Bl	D + C	V2-ATTACTGTGCCACCTGGGACG	TACCTCGCCGGTAGG	AAGAAACTCTTTGG-J1/2
MF IIA	Hö	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CCTTAGC	AAGAAACTCTTTGG-J1/2
MF IIA	Hö	Bl	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CCTTAGC	AAGAAACTCTTTGG-J1/2
MF IIB	He	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
MF IIB	He	Bl	C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
LyPap	Zo	Sk	D	V2-ATTACTGTGCCACCTGGGACGG	—	ATTATTATAAGAAACTCTTTGG-J1/2
LyPap	Zo	Bl	D	V2-ATTACTGTGCCACCTGGGACGG	—	ATTATTATAAGAAACTCTTTGG-J1/2
pleoCTCL	Zw	Sk	D + C	V7p-ATTACTGTGCCACCTGGGA	ATTGCCCTTGGTGGTGGTTGGA	TTCAAGATATTTG-JP1
pleoCTCl	Zw	Bl	D + C	V7p-ATTACTGTGCCACCTGGGA	ATTGCCCTTGGTGGTGGTTGGA	TTCAAGATATTTG-JP1
MF IA	Ot	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
MF IA	Ot	Bl-1	D + C	V7p-ATTACTGTGCCAC	GACCTTTT	TTATTATAAGAAACTCTTTGG-J1/2
pleoCTCl	Le	Sk	D + C	V8-ATTACTGTGCCACCTGGGATAG	AA	ATTATAAGAAACTCTTTGG-J1/2
pleoCTCl	Le	Bl	D + C	V8-ATTACTGTGCCACC	CATTCATCTCCAATCCAA	AATTATTATAAGAAACTCTTTGG-J1/2
MF IA	Bo	Bl	C	V2-ATTACTGTGCCACCTGGGACG	TGCCGGGTTG	GAAACTCTTTGG-J1/2
MF IB	Ja	Sk	D	V2-ATTACTGTGCCACCTGGGACGGG	CTTGATACT	AGAAACTCTTTGG-J1/2
MF IB	La	Sk	C	V2-ATTACTGTGCCACCTGGGACGGG	CG	TTATTATAAGAAACTCTTTGG-J1/2
MF IB	Cz	Bl	D + C	V4-ATTACTGTGCCACCTGGGATGGG	CAA	ATTATAAGAAACTCTTTGG-J1/2
MF IB	Me	Bl	D + C	V7p-ATTACTGTGCCACCTGGGACAGG	CCCC	ATTATAAGAAACTCTTTGG-J1/2
LyPap	Ti	Bl	D + C	V7p-ATTACTGTGCCACCT	CCCTCGATGTATTATGGTG	TATTATAAGAAACTCTTTGG-J1/2
Cell line	JM	Cells	D (VG1)†	V8-ATTACTGTGCCACCTGG	AAATT	TTATTATAAGAAACTCTTTGG-J2
Cell line	PEER	Cells	D (VG9)†	V9-TACTACTGTGCCTT	CCGGCCCG	AAGAAACTCTTTGG-J2
T-ALL	Ra	Bl	D (VG2)†	V10-ACTACTGTGCTGCGTGG	GAGGGGT	TTATTATAAGAAACTCTTTGG-J1/2
Cell line	JM	Cells	D (VG2)†	V11-GGTGGTGTACCACTGTGCCTG	TCAGATCCTCACAGGGCGGGTT	TAAGAAACTCTTTGG-J1

Diagnosis	Patient No.	Site	Sequencing	V-Segment	N-Region^3-150	J-Segment

MF IA	Ma	Sk	D + C	V8-ATTACTGTGCCACCTGGGAT	GAGC	AATTATTATAAGAAACTCTTTGG-J1/2
MF IA	Ma	Bl	D + C	V8-ATTACTGTGCCACCTGGGAT	GAGC	AATTATTATAAGAAACTCTTTGG-J1/2
MF IB	Ha	Sk	D + C	V2-ATTACTGTGCCACCTGGGACG	TACCTCGCCGGTAGG	AAGAAACTCTTTGG-J1/2
MF IB	Ha	Bl	D + C	V2-ATTACTGTGCCACCTGGGACG	TACCTCGCCGGTAGG	AAGAAACTCTTTGG-J1/2
MF IIA	Hö	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CCTTAGC	AAGAAACTCTTTGG-J1/2
MF IIA	Hö	Bl	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CCTTAGC	AAGAAACTCTTTGG-J1/2
MF IIB	He	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
MF IIB	He	Bl	C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
LyPap	Zo	Sk	D	V2-ATTACTGTGCCACCTGGGACGG	—	ATTATTATAAGAAACTCTTTGG-J1/2
LyPap	Zo	Bl	D	V2-ATTACTGTGCCACCTGGGACGG	—	ATTATTATAAGAAACTCTTTGG-J1/2
pleoCTCL	Zw	Sk	D + C	V7p-ATTACTGTGCCACCTGGGA	ATTGCCCTTGGTGGTGGTTGGA	TTCAAGATATTTG-JP1
pleoCTCl	Zw	Bl	D + C	V7p-ATTACTGTGCCACCTGGGA	ATTGCCCTTGGTGGTGGTTGGA	TTCAAGATATTTG-JP1
MF IA	Ot	Sk	D + C	V2-ATTACTGTGCCACCTGGGACGGG	CTTGAGAC	TATAAGAAACTCTTTGG-J1/2
MF IA	Ot	Bl-1	D + C	V7p-ATTACTGTGCCAC	GACCTTTT	TTATTATAAGAAACTCTTTGG-J1/2
pleoCTCl	Le	Sk	D + C	V8-ATTACTGTGCCACCTGGGATAG	AA	ATTATAAGAAACTCTTTGG-J1/2
pleoCTCl	Le	Bl	D + C	V8-ATTACTGTGCCACC	CATTCATCTCCAATCCAA	AATTATTATAAGAAACTCTTTGG-J1/2
MF IA	Bo	Bl	C	V2-ATTACTGTGCCACCTGGGACG	TGCCGGGTTG	GAAACTCTTTGG-J1/2
MF IB	Ja	Sk	D	V2-ATTACTGTGCCACCTGGGACGGG	CTTGATACT	AGAAACTCTTTGG-J1/2
MF IB	La	Sk	C	V2-ATTACTGTGCCACCTGGGACGGG	CG	TTATTATAAGAAACTCTTTGG-J1/2
MF IB	Cz	Bl	D + C	V4-ATTACTGTGCCACCTGGGATGGG	CAA	ATTATAAGAAACTCTTTGG-J1/2
MF IB	Me	Bl	D + C	V7p-ATTACTGTGCCACCTGGGACAGG	CCCC	ATTATAAGAAACTCTTTGG-J1/2
LyPap	Ti	Bl	D + C	V7p-ATTACTGTGCCACCT	CCCTCGATGTATTATGGTG	TATTATAAGAAACTCTTTGG-J1/2
Cell line	JM	Cells	D (VG1)†	V8-ATTACTGTGCCACCTGG	AAATT	TTATTATAAGAAACTCTTTGG-J2
Cell line	PEER	Cells	D (VG9)†	V9-TACTACTGTGCCTT	CCGGCCCG	AAGAAACTCTTTGG-J2
T-ALL	Ra	Bl	D (VG2)†	V10-ACTACTGTGCTGCGTGG	GAGGGGT	TTATTATAAGAAACTCTTTGG-J1/2
Cell line	JM	Cells	D (VG2)†	V11-GGTGGTGTACCACTGTGCCTG	TCAGATCCTCACAGGGCGGGTT	TAAGAAACTCTTTGG-J1

Abbreviations: D, direct sequencing; C, sequencing after cloning; Sk, skin; Bl, blood; MF, Mycosis fungoides; SS, Sézary's syndrome; pleoCTCL, pleomorphic CTCL; LyPap, lymphomatoid papulosis.

F3-150

N region was determined according to Breit et al.46

F3-151

V primer used for sequencing.

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