PCR and Sequencing Primers for TCRγ V and J Genes
| Name . | Primed Segments . | Position . | Sequence (5′ to 3′) . |
|---|---|---|---|
| VG1* | Vγ1, 2, 3, 4, 5, 5p, 6p, 7p, 8 | 104-121 | CTCCATCCACTGGTACCT |
| VG9 | Vγ9 | 121-138 | ATTGGTATCGAGAGAGAC |
| VG2 | Vγ10, 11, B, (A) | 111-129/117-135 | CACTGGTACKKGCAGAAAC |
| JG12-a* | Jγ1, 2 | 27-44 | CAACAAGTGTTGTTCCAC |
| JG12-i | Jγ1, 2 | 20-37 | TGTTGTTCCACTGCCAAA |
| JGP12-a | JγP1, P2 | 31-48 | CTATGAGCYTAGTCCCTT |
| JGP12-i | JγP1, P2 | 16-35 | CCTTYWGCAAAYRTCTTGA |
| VGseq | Vγ1, 2, 3, 4, 5, 5p, 6p, 7p, 8 | 136-153 | AGRCCCCACAGCRTCTTC |
| Name . | Primed Segments . | Position . | Sequence (5′ to 3′) . |
|---|---|---|---|
| VG1* | Vγ1, 2, 3, 4, 5, 5p, 6p, 7p, 8 | 104-121 | CTCCATCCACTGGTACCT |
| VG9 | Vγ9 | 121-138 | ATTGGTATCGAGAGAGAC |
| VG2 | Vγ10, 11, B, (A) | 111-129/117-135 | CACTGGTACKKGCAGAAAC |
| JG12-a* | Jγ1, 2 | 27-44 | CAACAAGTGTTGTTCCAC |
| JG12-i | Jγ1, 2 | 20-37 | TGTTGTTCCACTGCCAAA |
| JGP12-a | JγP1, P2 | 31-48 | CTATGAGCYTAGTCCCTT |
| JGP12-i | JγP1, P2 | 16-35 | CCTTYWGCAAAYRTCTTGA |
| VGseq | Vγ1, 2, 3, 4, 5, 5p, 6p, 7p, 8 | 136-153 | AGRCCCCACAGCRTCTTC |
Adapted from Volkenandt et al.10