The Synergistic and Additive Effects of IL-15 and IL-12 on CB LAK Cytotoxicity Against Daudi
| IL-12 (U/mL) . | IL-15 (ng/mL) . | |||||
|---|---|---|---|---|---|---|
| . | 0 . | 0.1 . | 0.5 . | 1.0 . | 10 . | 100 . |
| 0 | 86 ± 24* | 234 ± 62 | 348 ± 58 | 823 ± 68 | 930 ± 57 | |
| 0.1 | 198 ± 34 | 313 ± 54 (+10%)† | 512 ± 48 (+19%) | 542 ± 44 (−1%) | 576 ± 51 (−44%) | 601 ± 76 (−47%) |
| 0.5 | 378 ± 49 | 460 ± 51 (−3%) | 519 ± 53 (−17%) | 574 ± 68 (−22%) | ND | ND |
| 1.0 | 435 ± 54 | 487 ± 56 (−7%) | 516 ± 63 (−23%) | 573 ± 75 (−27%) | 403 ± 60 (−68%) | 372 ± 75 (−76%) |
| 10 | 501 ± 54 | 375 ± 97 (−36%) | ND | 354 ± 92 (−57%) | 259 ± 77 (−80%) | 381 ± 60 (−78%) |
| IL-12 (U/mL) . | IL-15 (ng/mL) . | |||||
|---|---|---|---|---|---|---|
| . | 0 . | 0.1 . | 0.5 . | 1.0 . | 10 . | 100 . |
| 0 | 86 ± 24* | 234 ± 62 | 348 ± 58 | 823 ± 68 | 930 ± 57 | |
| 0.1 | 198 ± 34 | 313 ± 54 (+10%)† | 512 ± 48 (+19%) | 542 ± 44 (−1%) | 576 ± 51 (−44%) | 601 ± 76 (−47%) |
| 0.5 | 378 ± 49 | 460 ± 51 (−3%) | 519 ± 53 (−17%) | 574 ± 68 (−22%) | ND | ND |
| 1.0 | 435 ± 54 | 487 ± 56 (−7%) | 516 ± 63 (−23%) | 573 ± 75 (−27%) | 403 ± 60 (−68%) | 372 ± 75 (−76%) |
| 10 | 501 ± 54 | 375 ± 97 (−36%) | ND | 354 ± 92 (−57%) | 259 ± 77 (−80%) | 381 ± 60 (−78%) |
Abbreviation: ND, not done.
LAK cytotoxicity (lytic units) in Table 2 is presented as mean ± SEM (n = 10).
(%) indicates the effects (synergistic < 10%, not additive < 0%) of IL-15 and IL-12 in combination on LAK activity compared with the sum of each cytokine alone. All the data are normalized and calculated in the same way as in Table 1.