Quantitation and Comparison of Chemotactic and Chemokinetic Activities of SDF-1 and SLF on MO7e Cells
Definitions* . | SDF-1 . | SLF . |
---|---|---|
Maximum chemotactic activity (MCTA) (%)† | 32.2 | 22.6 |
Specific MCTA (%/ng/mL)‡ | 0.322 | 2.26 |
Maximum chemokinetic activity (MCKA) (%)§ | 1.6 | 16.3 |
Specific MCKA (%/ng/mL)∥ | 0.016 | 1.63 |
Chemotactic-chemokinetic index (CCI)¶ | 20 | 1.38 |
Relative chemokinetic activity (RCKA) (%)# | 5 | 72 |
Definitions* . | SDF-1 . | SLF . |
---|---|---|
Maximum chemotactic activity (MCTA) (%)† | 32.2 | 22.6 |
Specific MCTA (%/ng/mL)‡ | 0.322 | 2.26 |
Maximum chemokinetic activity (MCKA) (%)§ | 1.6 | 16.3 |
Specific MCKA (%/ng/mL)∥ | 0.016 | 1.63 |
Chemotactic-chemokinetic index (CCI)¶ | 20 | 1.38 |
Relative chemokinetic activity (RCKA) (%)# | 5 | 72 |
*All the calculations in this table were based on the data of checkerboard experiments as shown in Table 1. Net migration after subtraction of background migration should be used for calculation if the background level is high. Background migration is the cell migration occurring at zero concentration of a chemoattractant, and dependent on cell types and chemotaxis system used. In this experiment, the background is zero.
A chemoattractant's maximal ability to induce migration of a type of cells in a positive gradient. MCTA of SDF-1 (32.2%) was determined at 100 ng/mL of SDF-1 only in the lower chamber, and MCTA of SLF (22.6%) was determined at 10 ng/mL of SLF only in the lower chamber (see Table1 for these figures).
MCTA per ng/mL chemoattractant, eg, specific MCTA (0.322 %/ng/mL) of SDF-1 was calculated by dividing MCTA (32.2%) with the concentration of SDF-1 (100 ng/mL) used.
A chemoattractant's maximal ability to induce cell migration in a zero gradient formed by equal concentrations of the chemoattractant in both chambers. MCKAs of SDF-1 and SLF were determined at 100 ng/mL and 10 ng/mL, respectively in both the upper and lower chambers.
∥MCKA per ng/mL chemoattractant, eg, specific MCKA (1.63%/ng/mL) of SLF was calculated by dividing MCKA (16.3%) by the concentration of SLF (10 ng/mL) used.
¶The relative strength of the chemotactic activity compared with the chemokinetic activity of a chemoattractant for a type of cells. This equals MCTA divided by MCKA, eg, CCI (20) of SDF-1 was calculated by dividing MCTA (32.2%) by MCKA (1.6%).
#The relative chemokinetic activity of a chemoattractant for a type of cells. This equals the percentage of MCKA divided by MCTA, eg, RCKA (5%) of SDF-1 was calculated by dividing MCKA (1.6%) by MCTA (32.2%), and multiplying by 100.