Effects of SDF-1 and SLF on Migration of MO7e Cells Assessed by Checkerboard Assay
| SDF-1 Concentration (ng/mL) in Lower Chamber* . | SDF-1 Concentration (ng/mL) in Upper Chamber . | ||||
|---|---|---|---|---|---|
| 0 . | 1 . | 10 . | 100 . | 1000 . | |
| 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 0.3 | 0 | 0 | 0 | 0 |
| 10 | 13.4 | 6.6 | 0.9 | 0 | 0 |
| 100 | 32.2† | 31.2 | 1.7 | 1.6‡ | 0 |
| 1,000 | 14.4 | 14.3 | 11.8 | 4.2 | 0.6 |
| SLF Concentration (ng/mL) in Lower Chamber* | SLF Concentration (ng/mL) in Upper Chamber | ||||
| 0 | 0.1 | 1 | 10 | 100 | |
| 0 | 0 | 0 | 0 | 0.4 | 2.1 |
| 0.1 | 0.2 | 0 | 0.2 | 0.3 | 1.7 |
| 1 | 3.5 | 2.2 | 1.8 | 1.0 | 2.6 |
| 10 | 22.6§ | 23.5 | 20.4 | 16.3∥ | 1.7 |
| 100 | 6.0 | 7.5 | 5.9 | 5.1 | 2.6 |
| SDF-1 Concentration (ng/mL) in Lower Chamber* . | SDF-1 Concentration (ng/mL) in Upper Chamber . | ||||
|---|---|---|---|---|---|
| 0 . | 1 . | 10 . | 100 . | 1000 . | |
| 0 | 0 | 0 | 0 | 0 | 0 |
| 1 | 0.3 | 0 | 0 | 0 | 0 |
| 10 | 13.4 | 6.6 | 0.9 | 0 | 0 |
| 100 | 32.2† | 31.2 | 1.7 | 1.6‡ | 0 |
| 1,000 | 14.4 | 14.3 | 11.8 | 4.2 | 0.6 |
| SLF Concentration (ng/mL) in Lower Chamber* | SLF Concentration (ng/mL) in Upper Chamber | ||||
| 0 | 0.1 | 1 | 10 | 100 | |
| 0 | 0 | 0 | 0 | 0.4 | 2.1 |
| 0.1 | 0.2 | 0 | 0.2 | 0.3 | 1.7 |
| 1 | 3.5 | 2.2 | 1.8 | 1.0 | 2.6 |
| 10 | 22.6§ | 23.5 | 20.4 | 16.3∥ | 1.7 |
| 100 | 6.0 | 7.5 | 5.9 | 5.1 | 2.6 |
*Cells were added to the upper chamber and the indicated chemoattractants were added to either the lower or upper chamber. Results shown are the percentages of cells that migrated into the lower chamber and are representative of three independent experiments.
Figure used to determine MCTA of SDF-1 for Table 2.
Figure used to determine MCKA of SDF-1 for Table 2.
Figure used to determine MCTA of SLF for Table 2.
∥Figure used to determine MCKA of SLF for Table 2.