Analysis of the GCV Sensitivity of Committed and Mixed Progenitor Cells Derived From BM Cells of Control, HαIIb-tk, and MαIIb-tk Mice in Individual Semisolid Assays
| . | GCV . | CFU-MK . | BFU-E . | CFU-E . | GM-CFC . | CFU-mix . | CFU-GEMMK . |
|---|---|---|---|---|---|---|---|
| Control | 0 | 20 ± 2 | 24 ± 2 | 108 ± 7 | 98 ± 5 | 16 ± 2 | 7 ± 1 |
| 0.5 | 18 ± 3 | 24 ± 2 | 98 ± 11 | 86 ± 2 | 13 ± 1 | 5 ± 1 | |
| 1 | 12 ± 5 | 22 ± 2 | 84 ± 6 | 70 ± 4 | 9 ± 1 | 7 ± 1 | |
| 5 | 13 ± 4 | 24 ± 7 | 77 ± 8 | 71 ± 9 | 9 ± 1 | 6 ± 1 | |
| HαIIb-tk (1) | 0 | 17 ± 3 | 22 ± 2 | 98 ± 6 | 88 ± 13 | 9 ± 2 | 4 ± 1 |
| 0.5 | ND | ND | 90 ± 4 | ND | ND | ND | |
| 1 | 2 ± 2 | 1 ± 1 | 62 ± 10 | 46 ± 8 | 0 | 0 | |
| 5 | 1 ± 1 | 0 | 50 ± 10 | 3 ± 1 | 0 | 0 | |
| MαIIb-tk (1) | 0 | 24 ± 4 | 17 ± 2 | 70 ± 9 | 70 ± 2 | 11 ± 1 | 7 ± 1 |
| 0.5 | 3 ± 2 | 5 ± 1 | 43 ± 7 | 45 ± 3 | 3 ± 1 | 2 ± 1 | |
| 1 | 5 ± 3 | 3 ± 1 | 53 ± 11 | 28 ± 1 | 3 ± 1 | 1 ± 1 | |
| 5 | 3 ± 2 | 1 ± 1 | 56 ± 8 | 10 ± 1 | 1 ± 1 | 0 ± 1 | |
| MαIIb-tk (3 or 4) | 0 | 20 ± 2 | 23 ± 4 | 93 ± 17 | 116 ± 7 | 6 ± 1 | 3 ± 1 |
| 0.5 | 0 ± 1 | 0 ± 1 | 34 ± 4 | 5 ± 1 | 0 | 0 | |
| 1 | 0 | 0 | 11 ± 2 | 0 | 0 | 0 | |
| 5 | 0 | 0 | 3 ± 1 | 0 | 0 | 0 |
| . | GCV . | CFU-MK . | BFU-E . | CFU-E . | GM-CFC . | CFU-mix . | CFU-GEMMK . |
|---|---|---|---|---|---|---|---|
| Control | 0 | 20 ± 2 | 24 ± 2 | 108 ± 7 | 98 ± 5 | 16 ± 2 | 7 ± 1 |
| 0.5 | 18 ± 3 | 24 ± 2 | 98 ± 11 | 86 ± 2 | 13 ± 1 | 5 ± 1 | |
| 1 | 12 ± 5 | 22 ± 2 | 84 ± 6 | 70 ± 4 | 9 ± 1 | 7 ± 1 | |
| 5 | 13 ± 4 | 24 ± 7 | 77 ± 8 | 71 ± 9 | 9 ± 1 | 6 ± 1 | |
| HαIIb-tk (1) | 0 | 17 ± 3 | 22 ± 2 | 98 ± 6 | 88 ± 13 | 9 ± 2 | 4 ± 1 |
| 0.5 | ND | ND | 90 ± 4 | ND | ND | ND | |
| 1 | 2 ± 2 | 1 ± 1 | 62 ± 10 | 46 ± 8 | 0 | 0 | |
| 5 | 1 ± 1 | 0 | 50 ± 10 | 3 ± 1 | 0 | 0 | |
| MαIIb-tk (1) | 0 | 24 ± 4 | 17 ± 2 | 70 ± 9 | 70 ± 2 | 11 ± 1 | 7 ± 1 |
| 0.5 | 3 ± 2 | 5 ± 1 | 43 ± 7 | 45 ± 3 | 3 ± 1 | 2 ± 1 | |
| 1 | 5 ± 3 | 3 ± 1 | 53 ± 11 | 28 ± 1 | 3 ± 1 | 1 ± 1 | |
| 5 | 3 ± 2 | 1 ± 1 | 56 ± 8 | 10 ± 1 | 1 ± 1 | 0 ± 1 | |
| MαIIb-tk (3 or 4) | 0 | 20 ± 2 | 23 ± 4 | 93 ± 17 | 116 ± 7 | 6 ± 1 | 3 ± 1 |
| 0.5 | 0 ± 1 | 0 ± 1 | 34 ± 4 | 5 ± 1 | 0 | 0 | |
| 1 | 0 | 0 | 11 ± 2 | 0 | 0 | 0 | |
| 5 | 0 | 0 | 3 ± 1 | 0 | 0 | 0 |
Marrow cells (5 × 104 per well) were cultured in 0.33 mL complete IMDM supplemented with predetermined optimal concentrations of cytokines as described in Materials and Methods.
Abbreviation: ND, not determined.