Primer Sequences
| Fragment Amplified . | Primers . |
|---|---|
| CYBB cDNA exons 4-10 | S 5′-AAGAGTTCGAAGACAACTGGACAGG-3′ |
| AS 5′-GAACACATCTTCACTGGCAGTGCCAAAGGGCCCATC-3′ | |
| CYBB gDNA exon 4exon 5 | S 5′-AAGAGTTCGAAGACAACTGGACAGG-3′ |
| AS 5′-GGATCAGAATTATTGACTCGGGCATTC-3′ | |
| CYBB gDNA exon 5exon 6 | S 5′-AGTAGCACTCTCTGAACTTG-3′ |
| AS 5′-GTACCAAAAGACTTCAAAGTAAGACCTCCGGATG-3′ | |
| CYBB gDNA intron 5intron 5 | S 5′-AAAAGTCAATCCTAGTGTC-3′ |
| AS 5′-GTGTCCTATGTATAGAACAATAG-3′ | |
| CYBB gDNA exon 6exon 7 | S 5′-TTTGTGATCTTCTTCATTGGCCTTGCC-3′ |
| AS 5′-GCACAGCCAAACTCTCTGCGGTCTGC-3′ | |
| CYBB gDNA exon 7exon 8 | S 5′-CTCAGTTTGCTGGAAACCCT-3′ |
| AS 5′-CTGTTGAGATCGCCAAAACCG-3′ | |
| CYBB gDNA exon 7intron 7 | S 5′-CTCAGTTTGCTGCAAACCCT-3′ |
| AS 5′-CACTGTGACAATTGCAAGATGAC-3′ |
| Fragment Amplified . | Primers . |
|---|---|
| CYBB cDNA exons 4-10 | S 5′-AAGAGTTCGAAGACAACTGGACAGG-3′ |
| AS 5′-GAACACATCTTCACTGGCAGTGCCAAAGGGCCCATC-3′ | |
| CYBB gDNA exon 4exon 5 | S 5′-AAGAGTTCGAAGACAACTGGACAGG-3′ |
| AS 5′-GGATCAGAATTATTGACTCGGGCATTC-3′ | |
| CYBB gDNA exon 5exon 6 | S 5′-AGTAGCACTCTCTGAACTTG-3′ |
| AS 5′-GTACCAAAAGACTTCAAAGTAAGACCTCCGGATG-3′ | |
| CYBB gDNA intron 5intron 5 | S 5′-AAAAGTCAATCCTAGTGTC-3′ |
| AS 5′-GTGTCCTATGTATAGAACAATAG-3′ | |
| CYBB gDNA exon 6exon 7 | S 5′-TTTGTGATCTTCTTCATTGGCCTTGCC-3′ |
| AS 5′-GCACAGCCAAACTCTCTGCGGTCTGC-3′ | |
| CYBB gDNA exon 7exon 8 | S 5′-CTCAGTTTGCTGGAAACCCT-3′ |
| AS 5′-CTGTTGAGATCGCCAAAACCG-3′ | |
| CYBB gDNA exon 7intron 7 | S 5′-CTCAGTTTGCTGCAAACCCT-3′ |
| AS 5′-CACTGTGACAATTGCAAGATGAC-3′ |
Primers for allele-specific PCR (Fig 4). Primer 1 S 5′-AAAAGTCAATCCTAGTGTC-3′; primer 2 AS 5′-GTGTCCTATGTATAGAACAATAG-3′; primer 3 S 5′-CAGTTCACATTTCCTATGACATC-3′; primer 4 AS 5′-CACTGTGACAATTGCAAGATGAC-3′.