Analysis of Survival Cohorts of Wild-Type, G−/−, GM−/−, and G−/−GM−/− Mice
| Genotype . | No. of Deaths . | Mean Age at Death (wk)3-152 . | Apparent Primary Cause . | Infections Encountered . | Other Findings . |
|---|---|---|---|---|---|
| . | (no. examined)3-150 . | . | of Death (no. of mice) . | (% animals affected) . | . |
| Wild-type | 8 (4) | 44 ± 20 | Bowel tumor (2) | None evident | |
| Renal cyst (1) | |||||
| No cause evident (1) | |||||
| G−/− | 31 (24) | 53 ± 16 | Infection and amyloid (10) | Any infection (63%) | No pulmonary infections found |
| Infection alone (5) | Superficial cellulitis (38%) | Amyloidosis in 54% | |||
| No cause evident (4) | Abscess formation (hepatic, intraabdominal, or perianal) (25%) | Thickening of alveolar septae in 79% | |||
| Tumors (lymphoma, soft-tissue, pancreatic) (3) | Bacterial isolates: S aureus × 1, P mirabilis × 1, Pasturella haemolytica × 1, Enterococci × 1 | ||||
| Amyloid without infection (2) | |||||
| GM−/− | 29 (19) | 66 ± 12 | Proteinosis and lung infection (8) | Any infection (84%) | All animals had lung proteinosis3-151 |
| Extrapulmonary infection (7) | Pulmonary infection (53%) | Amyloidosis in 16% | |||
| Lung proteinosis alone (3) | Soft-tissue infections (42%) | ||||
| Tumor (uterine) (1) | Bacterial isolates: S aureus × 1, Pasturella pneumotropica × 3, Streptococcus sp. × 2 | ||||
| G−/−GM−/− | 43 (25) | 49 ± 19 | Extrapulmonary infection (8) | Any infection (64%) | All animals had lung proteinosis; lymphoid accumulation more severe and widespread than in GM−/− 3-151 |
| Proteinosis and lung infection (7) | Pulmonary infection (36%) | Thickening of alveolar septae in 33% | |||
| Lung proteinosis alone (6) | Soft-tissue infection (36%) | Amyloidosis in 25% | |||
| No cause evident (3) | Bacterial isolates: S aureus × 1 | ||||
| Tumor (lung) (1) |
| Genotype . | No. of Deaths . | Mean Age at Death (wk)3-152 . | Apparent Primary Cause . | Infections Encountered . | Other Findings . |
|---|---|---|---|---|---|
| . | (no. examined)3-150 . | . | of Death (no. of mice) . | (% animals affected) . | . |
| Wild-type | 8 (4) | 44 ± 20 | Bowel tumor (2) | None evident | |
| Renal cyst (1) | |||||
| No cause evident (1) | |||||
| G−/− | 31 (24) | 53 ± 16 | Infection and amyloid (10) | Any infection (63%) | No pulmonary infections found |
| Infection alone (5) | Superficial cellulitis (38%) | Amyloidosis in 54% | |||
| No cause evident (4) | Abscess formation (hepatic, intraabdominal, or perianal) (25%) | Thickening of alveolar septae in 79% | |||
| Tumors (lymphoma, soft-tissue, pancreatic) (3) | Bacterial isolates: S aureus × 1, P mirabilis × 1, Pasturella haemolytica × 1, Enterococci × 1 | ||||
| Amyloid without infection (2) | |||||
| GM−/− | 29 (19) | 66 ± 12 | Proteinosis and lung infection (8) | Any infection (84%) | All animals had lung proteinosis3-151 |
| Extrapulmonary infection (7) | Pulmonary infection (53%) | Amyloidosis in 16% | |||
| Lung proteinosis alone (3) | Soft-tissue infections (42%) | ||||
| Tumor (uterine) (1) | Bacterial isolates: S aureus × 1, Pasturella pneumotropica × 3, Streptococcus sp. × 2 | ||||
| G−/−GM−/− | 43 (25) | 49 ± 19 | Extrapulmonary infection (8) | Any infection (64%) | All animals had lung proteinosis; lymphoid accumulation more severe and widespread than in GM−/− 3-151 |
| Proteinosis and lung infection (7) | Pulmonary infection (36%) | Thickening of alveolar septae in 33% | |||
| Lung proteinosis alone (6) | Soft-tissue infection (36%) | Amyloidosis in 25% | |||
| No cause evident (3) | Bacterial isolates: S aureus × 1 | ||||
| Tumor (lung) (1) |
Animals found dead were not subject to histologic or microbiologic analysis.
Both the mean severity and extent of lymphoid perivascular accumulation were greater in G−/−GM−/− mice than in GM−/− mice (3.0 v 2.1 and 79% v 44%, respectively, both P ≤ .0004), whereas the mean severity and extent of surfactant accumulation were not significantly different in G−/−GM−/− and GM−/− animals at the time of death (2.7 v 2.7 and 73% v 83%, respectively, both P > .1).
Mean age ± 1 SD of all animals dying, regardless of whether found dead or killed.