Colony-Forming Cells in the Bone Marrow and Spleen of Wild-Type, G−/−, GM−/−, and G−/−GM−/− Mice
| Cell Source . | Stimulus . | Genotype . | Total . | Granulocyte . | Macrophage . | Granulocyte/Macrophage . |
|---|---|---|---|---|---|---|
| Bone marrow | SCM | Wild-type | 48 ± 5 | 12 ± 8 | 12 ± 6 | 20 ± 3 |
| G−/− | 31 ± 12 | 7 ± 2 | 11 ± 7 | 10 ± 3* | ||
| GM−/− | 27 ± 14 | 5 ± 2 | 10 ± 6 | 11 ± 6 | ||
| G−/−GM−/− | 40 ± 8 | 9 ± 4 | 13 ± 6 | 15 ± 5 | ||
| GM-CSF | Wild-type | 53 ± 14 | 13 ± 7 | 13 ± 5 | 28 ± 10 | |
| G−/− | 24 ± 5* | 6 ± 1 | 6 ± 1* | 12 ± 4* | ||
| GM−/− | 34 ± 15 | 10 ± 2‡ | 8 ± 6 | 16 ± 10 | ||
| G−/−GM−/− | 32 ± 14 | 10 ± 6 | 4 ± 3* | 17 ± 6 | ||
| SCF | Wild-type | 25 ± 10 | 12 ± 7 | 1 ± 1 | 10 ± 5 | |
| G−/− | 21 ± 6 | 11 ± 4 | 1 ± 1 | 8 ± 5 | ||
| GM−/− | 18 ± 9 | 9 ± 2 | 0 ± 0‡ | 7 ± 6 | ||
| G−/−GM−/− | 19 ± 7 | 11 ± 3 | 1 ± 1 | 6 ± 3 | ||
| Spleen | SCM | Wild-type | 16 ± 11 | 2 ± 2 | 4 ± 3 | 7 ± 5 |
| G−/− | 10 ± 4 | 1 ± 1 | 2 ± 1 | 5 ± 2 | ||
| GM−/− | 31 ± 22‡ | 3 ± 2 | 7 ± 8 | 17 ± 10‡ | ||
| G−/−GM−/− | 8 ± 3† | 1 ± 0† | 2 ± 1 | 4 ± 2† | ||
| GM-CSF | Wild-type | 12 ± 6 | 2 ± 2 | 2 ± 2 | 8 ± 3 | |
| G−/− | 3 ± 3* | 0 ± 0 | 1 ± 2 | 2 ± 1* | ||
| GM−/− | 19 ± 12‡ | 4 ± 3 | 1 ± 1 | 13 ± 8‡ | ||
| G−/−GM−/− | 3 ± 2*† | 1 ± 1 | 0 ± 0*† | 2 ± 2*† | ||
| SCF | Wild-type | 11 ± 8 | 5 ± 4 | 1 ± 1 | 4 ± 4 | |
| G−/− | 6 ± 3 | 2 ± 2 | 1 ± 0 | 3 ± 2 | ||
| GM−/− | 26 ± 21‡ | 12 ± 11 | 2 ± 2 | 4 ± 4 | ||
| G−/−GM−/− | 3 ± 2* | 1 ± 1 | 0 ± 1 | 1 ± 1*† | ||
| SCF + GM-CSF | Wild-type | 28 ± 14 | 6 ± 3 | 3 ± 1 | 18 ± 11 | |
| G−/− | 17 ± 11 | 2 ± 1* | 2 ± 2 | 13 ± 10 | ||
| GM−/− | 62 ± 38‡ | 7 ± 5‡ | 5 ± 1*‡ | 45 ± 31‡ | ||
| G−/−GM−/− | 10 ± 6*† | 2 ± 1 | 2 ± 1† | 6 ± 4† |
| Cell Source . | Stimulus . | Genotype . | Total . | Granulocyte . | Macrophage . | Granulocyte/Macrophage . |
|---|---|---|---|---|---|---|
| Bone marrow | SCM | Wild-type | 48 ± 5 | 12 ± 8 | 12 ± 6 | 20 ± 3 |
| G−/− | 31 ± 12 | 7 ± 2 | 11 ± 7 | 10 ± 3* | ||
| GM−/− | 27 ± 14 | 5 ± 2 | 10 ± 6 | 11 ± 6 | ||
| G−/−GM−/− | 40 ± 8 | 9 ± 4 | 13 ± 6 | 15 ± 5 | ||
| GM-CSF | Wild-type | 53 ± 14 | 13 ± 7 | 13 ± 5 | 28 ± 10 | |
| G−/− | 24 ± 5* | 6 ± 1 | 6 ± 1* | 12 ± 4* | ||
| GM−/− | 34 ± 15 | 10 ± 2‡ | 8 ± 6 | 16 ± 10 | ||
| G−/−GM−/− | 32 ± 14 | 10 ± 6 | 4 ± 3* | 17 ± 6 | ||
| SCF | Wild-type | 25 ± 10 | 12 ± 7 | 1 ± 1 | 10 ± 5 | |
| G−/− | 21 ± 6 | 11 ± 4 | 1 ± 1 | 8 ± 5 | ||
| GM−/− | 18 ± 9 | 9 ± 2 | 0 ± 0‡ | 7 ± 6 | ||
| G−/−GM−/− | 19 ± 7 | 11 ± 3 | 1 ± 1 | 6 ± 3 | ||
| Spleen | SCM | Wild-type | 16 ± 11 | 2 ± 2 | 4 ± 3 | 7 ± 5 |
| G−/− | 10 ± 4 | 1 ± 1 | 2 ± 1 | 5 ± 2 | ||
| GM−/− | 31 ± 22‡ | 3 ± 2 | 7 ± 8 | 17 ± 10‡ | ||
| G−/−GM−/− | 8 ± 3† | 1 ± 0† | 2 ± 1 | 4 ± 2† | ||
| GM-CSF | Wild-type | 12 ± 6 | 2 ± 2 | 2 ± 2 | 8 ± 3 | |
| G−/− | 3 ± 3* | 0 ± 0 | 1 ± 2 | 2 ± 1* | ||
| GM−/− | 19 ± 12‡ | 4 ± 3 | 1 ± 1 | 13 ± 8‡ | ||
| G−/−GM−/− | 3 ± 2*† | 1 ± 1 | 0 ± 0*† | 2 ± 2*† | ||
| SCF | Wild-type | 11 ± 8 | 5 ± 4 | 1 ± 1 | 4 ± 4 | |
| G−/− | 6 ± 3 | 2 ± 2 | 1 ± 0 | 3 ± 2 | ||
| GM−/− | 26 ± 21‡ | 12 ± 11 | 2 ± 2 | 4 ± 4 | ||
| G−/−GM−/− | 3 ± 2* | 1 ± 1 | 0 ± 1 | 1 ± 1*† | ||
| SCF + GM-CSF | Wild-type | 28 ± 14 | 6 ± 3 | 3 ± 1 | 18 ± 11 | |
| G−/− | 17 ± 11 | 2 ± 1* | 2 ± 2 | 13 ± 10 | ||
| GM−/− | 62 ± 38‡ | 7 ± 5‡ | 5 ± 1*‡ | 45 ± 31‡ | ||
| G−/−GM−/− | 10 ± 6*† | 2 ± 1 | 2 ± 1† | 6 ± 4† |
Four mice were studied per genotype with each cell source and stimulus. All comparisons were made using the Mann-Whitney U test. Results were rounded to the nearest whole number after analysis and are expressed as the mean ± 1 SD number of colony-forming cells of triplicate determinations per 25,000 unselected bone marrow cells and per 200,000 unselected spleen cells. The sum of granulocyte, macrophage, and granulocyte-macrophage colonies may not equal total colonies, as mixed-lineage (megakaryocytic and granulocytic/monocytic or eosinophilic and granulocytic/monocytic), pure blast, and pure megakaryocytic colonies were quantified separately but are not tabulated. The sum of these additional colony types constituted a mean of 5.0% ± 3.8% of total bone marrow colonies and 7.9% ± 5.8% of total spleen colonies, and none of the 3 genotypes studied differed significantly from the wild-type.
P < .05 v wild-type.
P < .05 v GM−/−.
P < .05 v G−/−.