Activity of Wild-Type and mutGATA γ-Promoters in Stably Transformed K562 Cells
| γ Promoter . | Enhancer . | No. NeoRColonies per 1 × 106 Cells . | Fold Enhancement . | ||
|---|---|---|---|---|---|
| Exp. No. 1 . | Exp. No. 2 . | Exp. No. 1 . | Exp. No. 2 . | ||
| WT | None | 33 | 78 | 1.0 | 1.0 |
| WT | 5′HS2 | 1,650 | 3,225 | 50.0 | 41.3 |
| mutGATA | None | 102 | 219 | 3.1 | 2.8 |
| mutGATA | 5′HS2 | 2,610 | 6,000 | 79.0 | 77.0 |
| γ Promoter . | Enhancer . | No. NeoRColonies per 1 × 106 Cells . | Fold Enhancement . | ||
|---|---|---|---|---|---|
| Exp. No. 1 . | Exp. No. 2 . | Exp. No. 1 . | Exp. No. 2 . | ||
| WT | None | 33 | 78 | 1.0 | 1.0 |
| WT | 5′HS2 | 1,650 | 3,225 | 50.0 | 41.3 |
| mutGATA | None | 102 | 219 | 3.1 | 2.8 |
| mutGATA | 5′HS2 | 2,610 | 6,000 | 79.0 | 77.0 |
Equal numbers of K562 cells were electroporated with plasmids containing wild-type or mutGATA γ promoters ± 5′HS2 enhancer driving the neomycin resistance (neoR) gene as described in Materials and Methods. The number of neoR colonies were counted after plating and growth in complete media plus 1 mg/mL G418 and 0.3% methylcellulose.