IL-1-Ra, But Not Anti-PDGF Antibodies Reduce Platelet-Stimulated IL-6 Production of SMC
| . | Not Prestimulated* . | TNF-Prestimulated . | ||||
|---|---|---|---|---|---|---|
| . | None . | IL-1-Ra† . | IL-1-Ra + Ab† . | None . | IL-1-Ra . | IL-1-Ra + Ab . |
| Addition to SMC . | IL-6 Activity (ng/mL) . | IL-6 Activity (ng/mL) . | ||||
| Medium | 0.180 ± 0.025 | 0.180 ± 0.000 | 0.180 ± 0.000 | 1.000 ± 0.250 | 0.970 ± 0.085 | 1.100 ± 0.056 |
| Medium + thrombin | 0.400 ± 0.055 | 0.600 ± 0.075 | 0.800 ± 0.000 | 3.800 ± 0.250 | 3.900 ± 0.600 | 3.600 ± 0.540 |
| Platelets | 2.000 ± 0.350 | 1.000 ± 0.000 | 1.100 ± 0.000 | 21.000 ± 3.600 | 2.100 ± 0.300 | 2.100 ± 0.300 |
| Platelets + thrombin | 33.000 ± 0.400 | 5.200 ± 0.690 | 5.200 ± 1.800 | 139.000 ± 12.000 | 5.800 ± 0.450 | 7.700 ± 1.450 |
| . | Not Prestimulated* . | TNF-Prestimulated . | ||||
|---|---|---|---|---|---|---|
| . | None . | IL-1-Ra† . | IL-1-Ra + Ab† . | None . | IL-1-Ra . | IL-1-Ra + Ab . |
| Addition to SMC . | IL-6 Activity (ng/mL) . | IL-6 Activity (ng/mL) . | ||||
| Medium | 0.180 ± 0.025 | 0.180 ± 0.000 | 0.180 ± 0.000 | 1.000 ± 0.250 | 0.970 ± 0.085 | 1.100 ± 0.056 |
| Medium + thrombin | 0.400 ± 0.055 | 0.600 ± 0.075 | 0.800 ± 0.000 | 3.800 ± 0.250 | 3.900 ± 0.600 | 3.600 ± 0.540 |
| Platelets | 2.000 ± 0.350 | 1.000 ± 0.000 | 1.100 ± 0.000 | 21.000 ± 3.600 | 2.100 ± 0.300 | 2.100 ± 0.300 |
| Platelets + thrombin | 33.000 ± 0.400 | 5.200 ± 0.690 | 5.200 ± 1.800 | 139.000 ± 12.000 | 5.800 ± 0.450 | 7.700 ± 1.450 |
*SMC were incubated in 24-well culture plates until they reached confluency, washed and prestimulated with medium (Not prestimulated) or TNF-α (50 ng/mL; TNF-prestimulated) for 4 hours. Subsequently the cells were washed and medium (None), IL-1-Ra, or IL-1-Ra and anti-PDGF antibody (IL-1-Ra + Ab) were added. Then medium, medium containing thrombin (1 U/mL), platelets, or platelets with thrombin were added (24 hours). Platelets were added at a ratio of 1,000 platelets per SMC. IL-6 activity was measured in 7TD1-assay. Three similar experiments were performed.
IL-1-Ra, 2 μg/mL; Ab, anti-PDGF antibody, 1:200.