Enhancement of IgG Production Via CD27 Triggering in the Presence of IL-10
| Culture Condition . | IgG (ng/mL) . | ||
|---|---|---|---|
| UF B Cells . | CD27+B Cells . | CD27− B Cells . | |
| Medium alone | ND | ND | ND |
| IL-10 | 286 ± 12 | 364 ± 17 | ND |
| IL-10 + mock transfectants | 247 ± 8 | 376 ± 23 | ND |
| IL-10 + CD70 transfectants | 1,139 ± 89 | 1,654 ± 98 | ND |
| IL-10 + IL-2 | 892 ± 67 | 1,267 ± 113 | ND |
| IL-10 + IL-2 + mock transfectants | 763 ± 104 | 1,192 ± 84 | ND |
| IL-10 + IL-2 + CD70 transfectants | 4,693 ± 236 | 7,975 ± 846 | ND |
| Culture Condition . | IgG (ng/mL) . | ||
|---|---|---|---|
| UF B Cells . | CD27+B Cells . | CD27− B Cells . | |
| Medium alone | ND | ND | ND |
| IL-10 | 286 ± 12 | 364 ± 17 | ND |
| IL-10 + mock transfectants | 247 ± 8 | 376 ± 23 | ND |
| IL-10 + CD70 transfectants | 1,139 ± 89 | 1,654 ± 98 | ND |
| IL-10 + IL-2 | 892 ± 67 | 1,267 ± 113 | ND |
| IL-10 + IL-2 + mock transfectants | 763 ± 104 | 1,192 ± 84 | ND |
| IL-10 + IL-2 + CD70 transfectants | 4,693 ± 236 | 7,975 ± 846 | ND |
Data are expressed as the mean ± SD for triplicate wells and are representative of 4 experiments using different donors. B cells were cultured at 1.25 × 105/mL for 8 days in the indicated culture conditions.
Abbreviations: UF, unfractionated; ND, not detected.