Inhibition of RBC Cold Agglutination Capacity of Anti-Sia-lb CAs, GAS, and Kn by Oligosaccharides
| Oligosaccharide . | Inhibition (mmol/L) . | ||
|---|---|---|---|
| Designation . | Structure . | GAS . | Kn . |
| 1.Sialyllactose | NeuNAcα2-3Galβ1-4Glc | 2.5 | 1.25 |
| 2.Sialyllactosamine | NeuNAcα2-3Galβ1-4GlcNAc | 1.25 | ND |
| 3.Sialylfucosyllactose | NeuNAcα2-3Galβ1-4[Fucα1-3]Glc | 1.25 | 1.25 |
| 4.sLex | NeuNAcα2-3Galβ1-4[Fucα1-3]GlcNAc | 1.25 | ND |
| 5.Nonfucosylated sLea | NeuNAcα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc | 1.25 | 0.63 |
| 6.sLea | NeuNAcα2-3Galβ1-3[Fucα1-4]GlcNAc | 1.25 | ND |
| 7. | Galβ1-3GalNAcβ1-4[NeuNAcα2-3]Galβ1-4Glc | 0.63 | 1.25 |
| 8.Lex | Galβ1-4[Fucα1-3]GlcNAc | NI | NI |
| 9.Lea | Galβ1-3[Fucα1-4]GlcNAc | NI | NI |
| Oligosaccharide . | Inhibition (mmol/L) . | ||
|---|---|---|---|
| Designation . | Structure . | GAS . | Kn . |
| 1.Sialyllactose | NeuNAcα2-3Galβ1-4Glc | 2.5 | 1.25 |
| 2.Sialyllactosamine | NeuNAcα2-3Galβ1-4GlcNAc | 1.25 | ND |
| 3.Sialylfucosyllactose | NeuNAcα2-3Galβ1-4[Fucα1-3]Glc | 1.25 | 1.25 |
| 4.sLex | NeuNAcα2-3Galβ1-4[Fucα1-3]GlcNAc | 1.25 | ND |
| 5.Nonfucosylated sLea | NeuNAcα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc | 1.25 | 0.63 |
| 6.sLea | NeuNAcα2-3Galβ1-3[Fucα1-4]GlcNAc | 1.25 | ND |
| 7. | Galβ1-3GalNAcβ1-4[NeuNAcα2-3]Galβ1-4Glc | 0.63 | 1.25 |
| 8.Lex | Galβ1-4[Fucα1-3]GlcNAc | NI | NI |
| 9.Lea | Galβ1-3[Fucα1-4]GlcNAc | NI | NI |
Values correspond to the concentration (in millimoles per liter) inhibiting RBC cold agglutination. Oligosaccharides were obtained from Sigma (no. 1), from BioCarb Chemicals (Lund, Sweden) (nos. 2, 3, 5, and 7), and from Oxford Glycosystems Ltd (Oxford, UK) (nos. 4, 6, 8, and 9).
Abbreviations: NI, not inhibitory at 10 mmol/L; ND, not done.