Effect of IL-1α and IL-3 on the Formation of NK Cell Colonies From Fetal Liver Cells
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| IL-2, IL-7, IL-11, SF | 7 ± 1 | 1 ± 1 | 2 ± 1 | 9 ± 3 |
| IL-1α, IL-2, IL-7, IL-11, SF | 0 | 0 | 0 | 14 ± 2 |
| IL-3, IL-2, IL-7, IL-11, SF | 0 | 0 | 0 | 18 ± 4 |
| Cytokines . | No. of Colonies . | |||
|---|---|---|---|---|
| . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| IL-2, IL-7, IL-11, SF | 7 ± 1 | 1 ± 1 | 2 ± 1 | 9 ± 3 |
| IL-1α, IL-2, IL-7, IL-11, SF | 0 | 0 | 0 | 14 ± 2 |
| IL-3, IL-2, IL-7, IL-11, SF | 0 | 0 | 0 | 18 ± 4 |
Sca-1+c-kit+ cells were purified from fetal liver. A total of 50 cells was plated in methylcellulose media in the presence of designated cytokines. Colonies were scored after 18 days of incubation. Data represent mean ± SD of quadruplicate cultures.