Effects of Addition of IL-11 on the Colony Formation From Fetal Liver and Fetal Thymus Cells
| Cell Plated . | Cytokines . | No. of Colonies . | ||||
|---|---|---|---|---|---|---|
| Type . | No./Dish . | . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| Sca-1+c-kit+ FL | 50 | IL-2, IL-7, SF | 0 | 1 ± 1 | 3 ± 1 | 9 ± 1 |
| IL-2, IL-7, IL-11, SF | 7 ± 1 | 2 ± 1 | 4 ± 1 | 9 ± 2 | ||
| Unfractionated FT | 1000 | IL-2, IL-7, SF | 0 | 11 ± 1 | 0 | 2 ± 1 |
| IL-2, IL-7, IL-11, SF | 0 | 13 ± 1 | 0 | 3 ± 2 | ||
| CD25−c-kit+FT | 300 | IL-2, IL-7, SF | 0 | 22 ± 3 | 0 | 1 ± 1 |
| IL-2, IL-7, IL-11, SF | 0 | 18 ± 2 | 0 | 2 ± 1 | ||
| Cell Plated . | Cytokines . | No. of Colonies . | ||||
|---|---|---|---|---|---|---|
| Type . | No./Dish . | . | Mixed-NK . | Pure-NK . | Pre-B . | Myeloid . |
| Sca-1+c-kit+ FL | 50 | IL-2, IL-7, SF | 0 | 1 ± 1 | 3 ± 1 | 9 ± 1 |
| IL-2, IL-7, IL-11, SF | 7 ± 1 | 2 ± 1 | 4 ± 1 | 9 ± 2 | ||
| Unfractionated FT | 1000 | IL-2, IL-7, SF | 0 | 11 ± 1 | 0 | 2 ± 1 |
| IL-2, IL-7, IL-11, SF | 0 | 13 ± 1 | 0 | 3 ± 2 | ||
| CD25−c-kit+FT | 300 | IL-2, IL-7, SF | 0 | 22 ± 3 | 0 | 1 ± 1 |
| IL-2, IL-7, IL-11, SF | 0 | 18 ± 2 | 0 | 2 ± 1 | ||
Sca-1+c-kit+FL cells were prepared as described in the Materials and Methods section. CD25−c-kit+FT cells were prepared by FACS sorting. Cells were cultured in methylcellulose media in the presence of designated cytokines. Colonies were scored after 18 days of incubation. Data represent mean ± SD of quadruplicate cultures.
Abbreviations: Mixed-NK, mixed NK colonies; Pure-NK, NK cell colonies; Pre-B, Pre-B cell colonies; Myeloid, colonies consisting of mast cells, macrophages or both; FL, fetal liver; FT; fetal thymus.