Engraftment of Purified Allogeneic CD34+ Lymphocyte-Depleted Marrow Cells
| Animal . | CD34+/kg . | Days After Transplant . | Survival (yr) . | ||||
|---|---|---|---|---|---|---|---|
| . | (×10−6) . | WBC* . | ANC† >500 . | PLTS‡ >20,000 . | % Donorρ on d 28 . | . | |
| . | . | >500 . | >1,000 . | . | . | . | . |
| A89164 | 1.8 | 20 | 24 | 28 | 23 | 90% | 5 |
| A89163 | 2.1 | 17 | 20 | 22 | 28 | 50% | >6.0 |
| A91382 | 1.9 | 14 | 17 | 17 | 14 | 80% | >4.9 |
| Animal . | CD34+/kg . | Days After Transplant . | Survival (yr) . | ||||
|---|---|---|---|---|---|---|---|
| . | (×10−6) . | WBC* . | ANC† >500 . | PLTS‡ >20,000 . | % Donorρ on d 28 . | . | |
| . | . | >500 . | >1,000 . | . | . | . | . |
| A89164 | 1.8 | 20 | 24 | 28 | 23 | 90% | 5 |
| A89163 | 2.1 | 17 | 20 | 22 | 28 | 50% | >6.0 |
| A91382 | 1.9 | 14 | 17 | 17 | 14 | 80% | >4.9 |
The purified allogeneic CD34+ marrow cells devoid of detectable mature and immature lymphocytes and myeloid cells were greater than 95%, greater than 97%, and greater than 98% CD34+ at the time of transplantation for animals A89164, A89163, and A91382, respectively.
Day after which total white blood cell count was maintained above 500/μL and 1,000/μL.
Day after which absolute neutrophil count was maintained above 500/μL.
Day after which platelet count was maintained above 20,000/μl without transfusion.
ρ Karyotyping was performed on bone marrow cells from each animal on day +28. For A89164, 18 of 20 metaphases were female (donor); for A89163, 8 or 16 metaphases were male (donor); and for A91382, 16 of 20 metaphases were male (donor).