Frequency of NOD/Scid Mice Engrafted and Not Engrafted at Various Doses of Human Hematopoietic Cells
| CD34+ Cell Dose × 103 . | Drug Exposure After PBPC Enrichment . | No. of Positive Mice . | No. of Negative Mice . |
|---|---|---|---|
| 1,000 | no | 5 | 1 |
| 1-1,000 | 5-FU, SCF, IL-3 | 10 | 3 |
| 0.1-1 | 5-FU, SCF, IL-3 | 0 | 2 |
| 1-1,000 | mafosfamide and taxol | 5 | 0 |
| 0.1-1 | mafosfamide and taxol | 10 | 9 |
| CD34+ Cell Dose × 103 . | Drug Exposure After PBPC Enrichment . | No. of Positive Mice . | No. of Negative Mice . |
|---|---|---|---|
| 1,000 | no | 5 | 1 |
| 1-1,000 | 5-FU, SCF, IL-3 | 10 | 3 |
| 0.1-1 | 5-FU, SCF, IL-3 | 0 | 2 |
| 1-1,000 | mafosfamide and taxol | 5 | 0 |
| 0.1-1 | mafosfamide and taxol | 10 | 9 |
Mice were considered to be engrafted if human cells were detected by flow cytometry and a band was detectable by PCR analysis 6 to 10 weeks after transplantation.
Abbreviations: PBPC, peripheral blood progenitor cell; FU, fluorouracil; SCF, stem cell factor; IL, interleukin; PCR, polymerase chain reaction.