Table 2. Direct Effect of G-CSF on NK... | American Society of Hematology

Table 2.

Direct Effect of G-CSF on NK Proliferation and Cytotoxicity

G-CSF Concentration (ng/mL)	Proliferation	No IL-2 Resting Cytotoxicity	IL-2–Stimulated Cytotoxicity
	(% of control)^*	(% specific lysis)^†	(% specific lysis)^†
0	100	54 ± 2.4	60 ± 2.7
5	105 ± 2.9	ND	ND
10	96 ± 6.3	54 ± 1.1	75 ± 4.6
50	93 ± 3.2	56 ± 2.0	71 ± 2.1
100	79 ± 6.0^‡	54 ± 2.5	70 ± 1.2

G-CSF Concentration (ng/mL)	Proliferation	No IL-2 Resting Cytotoxicity	IL-2–Stimulated Cytotoxicity
	(% of control)^*	(% specific lysis)^†	(% specific lysis)^†
0	100	54 ± 2.4	60 ± 2.7
5	105 ± 2.9	ND	ND
10	96 ± 6.3	54 ± 1.1	75 ± 4.6
50	93 ± 3.2	56 ± 2.0	71 ± 2.1
100	79 ± 6.0^‡	54 ± 2.5	70 ± 1.2

n = 3 donors in triplicate for all determinations.

Abbreviation: ND, not determined.

Determined after 48 hours of stimulation before thymidine pulse (G-CSF added at time 0).

Cytotoxicity presented at E:T ratio 2.2:1 against K562 after a 16-hour incubation before testing.

P = .075.