Antibodies Used for Immunohistochemical Staining
| Antibody . | Clone . | Isotype . | Dilution . | Source . |
|---|---|---|---|---|
| Anti–B7-1 (CD80) | EW3.4B2.C4 | IgG2a | 1:250 | Repligen |
| A5.F5 | IgG2a | 1:500 | Repligen | |
| 104 | IgG1 | 1:500 | Coulter | |
| Anti–B7-2 (CD86) | FUN-1 | IgG1 | 1:2,000 | Pharmingen |
| HA5.2B7 | IgG2b | 1:2,000 | Repligen | |
| Anti-CD20 | B1 | IgG2a | 1:4,000 | Coulter |
| Anti-CD3 | UCHT1 | IgG1 | 1:2,000 | Dako |
| Anti-κ | A8B5 | IgG1 | 1:50 | Dako |
| Anti-λ | N10/2 | IgG1 | 1:50 | Dako |
| Antibody . | Clone . | Isotype . | Dilution . | Source . |
|---|---|---|---|---|
| Anti–B7-1 (CD80) | EW3.4B2.C4 | IgG2a | 1:250 | Repligen |
| A5.F5 | IgG2a | 1:500 | Repligen | |
| 104 | IgG1 | 1:500 | Coulter | |
| Anti–B7-2 (CD86) | FUN-1 | IgG1 | 1:2,000 | Pharmingen |
| HA5.2B7 | IgG2b | 1:2,000 | Repligen | |
| Anti-CD20 | B1 | IgG2a | 1:4,000 | Coulter |
| Anti-CD3 | UCHT1 | IgG1 | 1:2,000 | Dako |
| Anti-κ | A8B5 | IgG1 | 1:50 | Dako |
| Anti-λ | N10/2 | IgG1 | 1:50 | Dako |
Dako location: Carpinteria, CA.