Synthetic Oligonucleotides, Primers, and Probe for Amplification of BCR/ABL mRNA and cDNA, β-Actin mRNA, and AS mRNA
| . | Purpose . | . | Sequence . |
|---|---|---|---|
| Modified AS sequence | 5′-GCTCTCTATAGAAGGGCTTTTGAACTCTGCGAAGGGCTTTTGAACTCTGC-3′ | ||
| AS mRMA | RT-PCR | Primer A | 5′-GCAAGCTTCTCGAGGCTCTCTATAGAAGG-3′ |
| Primer B | 5′-CTGCAGGCAGAGTTCAA-3′ | ||
| Probe C | 5′-GAAGGGCTTTTGAACTCTGCGAAGGGG-3′ | ||
| b3a2 BCR/ABL mRNA | RT-PCR | Primer D | 5′-GGAGCTGCAGATGCTGACCAAC-3′ |
| Primer E | 5′-TCAGACCCTGAGGCTCAAAGTC-3′ | ||
| Probe F | 5′-GCTGAAGGGCTTTTGAACTCTGCTTA-3′ | ||
| ela2 BCR/ABL mRNA | RT-PCR | Primer G | 5′-GTTGTCGTGTCCGAGGCC-3′ |
| Primer H | 5′-TCAGACCCTGAGGCTCAAAGTC-3′ | ||
| Probe I | 5′-GGCCGCTGAAGGGCTTCTGCG-3′ | ||
| β-actin mRNA | RT-PCR | Primer J | 5′-TACCTCATGAAGATCCTCA-3′ |
| Primer K | 5′-TTCGTGGATGCCACAGGAC-3′ | ||
| Probe L | 5′-CCATCTCTTGCTCGAAGTC-3′ |
| . | Purpose . | . | Sequence . |
|---|---|---|---|
| Modified AS sequence | 5′-GCTCTCTATAGAAGGGCTTTTGAACTCTGCGAAGGGCTTTTGAACTCTGC-3′ | ||
| AS mRMA | RT-PCR | Primer A | 5′-GCAAGCTTCTCGAGGCTCTCTATAGAAGG-3′ |
| Primer B | 5′-CTGCAGGCAGAGTTCAA-3′ | ||
| Probe C | 5′-GAAGGGCTTTTGAACTCTGCGAAGGGG-3′ | ||
| b3a2 BCR/ABL mRNA | RT-PCR | Primer D | 5′-GGAGCTGCAGATGCTGACCAAC-3′ |
| Primer E | 5′-TCAGACCCTGAGGCTCAAAGTC-3′ | ||
| Probe F | 5′-GCTGAAGGGCTTTTGAACTCTGCTTA-3′ | ||
| ela2 BCR/ABL mRNA | RT-PCR | Primer G | 5′-GTTGTCGTGTCCGAGGCC-3′ |
| Primer H | 5′-TCAGACCCTGAGGCTCAAAGTC-3′ | ||
| Probe I | 5′-GGCCGCTGAAGGGCTTCTGCG-3′ | ||
| β-actin mRNA | RT-PCR | Primer J | 5′-TACCTCATGAAGATCCTCA-3′ |
| Primer K | 5′-TTCGTGGATGCCACAGGAC-3′ | ||
| Probe L | 5′-CCATCTCTTGCTCGAAGTC-3′ |