Table 1. Persistence of CD34 Expression... | American Society of Hematology

Table 1.

Persistence of CD34 Expression in Cultures Initiated With BM CD34⁺ Cells Isolated in Subcompartments of the G₀/G₁Phase of the Cell Cycle

Cultures Initiated on Day 0 With	% CD34⁺ Cells on Day 14	Total No. of CD34⁺ Cells on Day 14^‡
G₀CD34⁺	7.83 ± 0.85^*	29,383 ± 14,756
G_1aCD34⁺	4.31 ± 0.39^†	10,667 ± 2,462
G_1bCD34⁺	1.21 ± 0.57	6,167 ± 1,999
G_1cCD34⁺	0.53 ± 0.23	1,869 ± 1,255

Cultures Initiated on Day 0 With	% CD34⁺ Cells on Day 14	Total No. of CD34⁺ Cells on Day 14^‡
G₀CD34⁺	7.83 ± 0.85^*	29,383 ± 14,756
G_1aCD34⁺	4.31 ± 0.39^†	10,667 ± 2,462
G_1bCD34⁺	1.21 ± 0.57	6,167 ± 1,999
G_1cCD34⁺	0.53 ± 0.23	1,869 ± 1,255

A total of 10⁴ BM CD34⁺ cells isolated from G₀/G₁ subcompartments (Fig 4) were plated in complete medium supplemented with IL-3-IL-6-SCF. After 14 days, the persistence of CD34 expression was determined by FACS analysis of cultured cells. Data are the mean ± SEM, n = 3. Statistical analysis was by ANOVA followed by SNK pairwise comparisons.

P < .05 v cultures initiated with G_1a , G_1b , and G_1c CD34⁺ cells.

†

P < .05 v cultures initiated with G_1b and G_1c CD34⁺ cells.

‡

Total number of CD34⁺ cells calculated by multiplying the percentage of CD34⁺ cells by the total cell number present in each culture.

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