Colony Formation From CD34+ Cells Separated on the Basis of AC133 Expression
Fraction . | % of Population . | GM . | E . | CFU-Mix . | Total . |
---|---|---|---|---|---|
CD34+ | 1.6 | 15 ± 1 | 20 ± 4 | 8 ± 3 | 42 ± 4 |
CD34+AC133− | 1.3 | 13 ± 1 | 30 ± 5 | 16 ± 3 | 59 ± 7 |
CD34+AC133+ | 0.3 | 82 ± 8 | 24 ± 2 | 18 ± 2 | 124 ± 9 |
Fraction . | % of Population . | GM . | E . | CFU-Mix . | Total . |
---|---|---|---|---|---|
CD34+ | 1.6 | 15 ± 1 | 20 ± 4 | 8 ± 3 | 42 ± 4 |
CD34+AC133− | 1.3 | 13 ± 1 | 30 ± 5 | 16 ± 3 | 59 ± 7 |
CD34+AC133+ | 0.3 | 82 ± 8 | 24 ± 2 | 18 ± 2 | 124 ± 9 |
Five hundred cells were incubated in serum-free medium containing cytokines for 14 days. Colonies were identified in situ.3
Abbreviations: GM, granulocyte and/or macrophage colonies; E, erythroid colonies; CFU-Mix, mixed colonies containing erythroid and myeloid cells and/or megakaryocytes.