Table 3.

Colony Formation From CD34+ Cells Separated on the Basis of AC133 Expression

Fraction% of PopulationGMECFU-MixTotal
CD34+ 1.6 15 ± 1 20 ± 4 8 ± 3 42 ± 4 
CD34+AC133 1.3 13 ± 1 30 ± 5 16 ± 3 59 ± 7 
CD34+AC133+ 0.3 82 ± 8 24 ± 2 18 ± 2 124 ± 9 
Fraction% of PopulationGMECFU-MixTotal
CD34+ 1.6 15 ± 1 20 ± 4 8 ± 3 42 ± 4 
CD34+AC133 1.3 13 ± 1 30 ± 5 16 ± 3 59 ± 7 
CD34+AC133+ 0.3 82 ± 8 24 ± 2 18 ± 2 124 ± 9 

Five hundred cells were incubated in serum-free medium containing cytokines for 14 days. Colonies were identified in situ.3 

Abbreviations: GM, granulocyte and/or macrophage colonies; E, erythroid colonies; CFU-Mix, mixed colonies containing erythroid and myeloid cells and/or megakaryocytes.

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