Flow-Cytometric Determination of Differentiation of HL60, U937, and KG1 Induced by 72-Hours Incubation With ATRA, 1,25(OH)2 D3, and GM-CSF
| Cell . | Antigen . | Control . | GM-CSF . | ATRA . | ATRA + GM-CSF . | 1,25(OH)2 D3 . | 1,25(OH)2 D3 + GM-CSF . |
|---|---|---|---|---|---|---|---|
| HL60 | CD13 | 99.88 (0.1) | 99.87 (0.1) | 99.95 (0.1) | 99.93 (0.1) | 99.90 (0.1) | 99.93 (0.1) |
| 85.53 (19.6) | 76.13 (12.9) | 37.13 (8.7)* | 42.90 (15.0) | 65.63 (20.2) | 66.97 (23.6) | ||
| HL60 | CD33 | 86.87 (9.0) | 94.35 (2.9) | 69.40 (11.8) | 71.2 (13.6) | 65.77 (3.7) | 66.15 (13.6) |
| 6.16 (2.4) | 7.51 (1.3) | 4.43 (1.6) | 5.41 (0.5) | 4.81 (2.1)* | 6.06 (1.1) | ||
| U937 | CD11b | 2.80 (0.7) | 5.07 (2.2) | 14.57 (4.7)* | 19.65 (10.2) | 9.27 (5.9) | 46.20 (22.1) |
| 3.49 (0.6) | 4.36 (1.3) | 3.14 (0.7) | 1.83 (0.1) | 4.64 (1.1) | 3.51 (0.5) | ||
| U937 | CD14 | 1.44 (0.4) | 11.98 (5.7)* | 1.80 (1.7) | 39.18 (8.5)† | 35.04 (19.9)* | 90.34 (18.1)† |
| 0 | 2.87 (0.7) | 2.25 (2.5) | 1.72 (0.7) | 2.98 (1.4) | 51.48 (10.8)† | ||
| U937 | CD32 | 23.98 (2.4) | 29.06 (12.7) | 8.56 (7.0) | 53.76 (20.6)† | 22.22 (14.1) | 54.88 (13.9)† |
| 5.26 (2.8) | 6.86 (3.4)* | 6.62 (4.4) | 5.32 (3.7) | 5.99 (3.1) | 7.23 (4.7) | ||
| KG1 | CD11b | 32.62 (5.9) | 33.77 (9.3) | 70.46 (11.5)* | 90.37 (3.0)† | 85.30 (8.6)* | 95.20 (1.2) |
| 4.87 (1.1) | 4.50 (0.8) | 4.95 (1.5) | 4.72 (0.4) | 9.63 (2.0)* | 10.08 (2.2) | ||
| KG1 | CD13 | 99.83 (0.1) | 100.00 (0) | 99.92 (0.1) | 99.93 (0.1) | 99.87 (0.1) | 99.90 (0.1) |
| 81.12 (47.7) | 139.57 (48.6) | 38.98 (16.0)* | 44.93 (9.2) | 59.13 (29.6) | 81.83 (18.8) | ||
| KG1 | CD33 | 53.90 (15.5) | 50.45 (12.9) | 68.28 (7.9) | 26.95 (3.0) | 26.35 (14.7) | 28.20 (12.6) |
| 4.45 (1.1) | 4.02 (1.1) | 2.93 (1.0)* | 3.02 (1.1) | 3.96 (1.6) | 3.18 (1.6) |
| Cell . | Antigen . | Control . | GM-CSF . | ATRA . | ATRA + GM-CSF . | 1,25(OH)2 D3 . | 1,25(OH)2 D3 + GM-CSF . |
|---|---|---|---|---|---|---|---|
| HL60 | CD13 | 99.88 (0.1) | 99.87 (0.1) | 99.95 (0.1) | 99.93 (0.1) | 99.90 (0.1) | 99.93 (0.1) |
| 85.53 (19.6) | 76.13 (12.9) | 37.13 (8.7)* | 42.90 (15.0) | 65.63 (20.2) | 66.97 (23.6) | ||
| HL60 | CD33 | 86.87 (9.0) | 94.35 (2.9) | 69.40 (11.8) | 71.2 (13.6) | 65.77 (3.7) | 66.15 (13.6) |
| 6.16 (2.4) | 7.51 (1.3) | 4.43 (1.6) | 5.41 (0.5) | 4.81 (2.1)* | 6.06 (1.1) | ||
| U937 | CD11b | 2.80 (0.7) | 5.07 (2.2) | 14.57 (4.7)* | 19.65 (10.2) | 9.27 (5.9) | 46.20 (22.1) |
| 3.49 (0.6) | 4.36 (1.3) | 3.14 (0.7) | 1.83 (0.1) | 4.64 (1.1) | 3.51 (0.5) | ||
| U937 | CD14 | 1.44 (0.4) | 11.98 (5.7)* | 1.80 (1.7) | 39.18 (8.5)† | 35.04 (19.9)* | 90.34 (18.1)† |
| 0 | 2.87 (0.7) | 2.25 (2.5) | 1.72 (0.7) | 2.98 (1.4) | 51.48 (10.8)† | ||
| U937 | CD32 | 23.98 (2.4) | 29.06 (12.7) | 8.56 (7.0) | 53.76 (20.6)† | 22.22 (14.1) | 54.88 (13.9)† |
| 5.26 (2.8) | 6.86 (3.4)* | 6.62 (4.4) | 5.32 (3.7) | 5.99 (3.1) | 7.23 (4.7) | ||
| KG1 | CD11b | 32.62 (5.9) | 33.77 (9.3) | 70.46 (11.5)* | 90.37 (3.0)† | 85.30 (8.6)* | 95.20 (1.2) |
| 4.87 (1.1) | 4.50 (0.8) | 4.95 (1.5) | 4.72 (0.4) | 9.63 (2.0)* | 10.08 (2.2) | ||
| KG1 | CD13 | 99.83 (0.1) | 100.00 (0) | 99.92 (0.1) | 99.93 (0.1) | 99.87 (0.1) | 99.90 (0.1) |
| 81.12 (47.7) | 139.57 (48.6) | 38.98 (16.0)* | 44.93 (9.2) | 59.13 (29.6) | 81.83 (18.8) | ||
| KG1 | CD33 | 53.90 (15.5) | 50.45 (12.9) | 68.28 (7.9) | 26.95 (3.0) | 26.35 (14.7) | 28.20 (12.6) |
| 4.45 (1.1) | 4.02 (1.1) | 2.93 (1.0)* | 3.02 (1.1) | 3.96 (1.6) | 3.18 (1.6) |