HLA-DR 1- and HLA-DR 3-Bound Peptides From CD34+ Blast Cells of a Patient With CML
| Precursor Protein . | Sequence/Anchors3-150 . | Predominant Distribution . | ||||
|---|---|---|---|---|---|---|
| . | i+3+8 . | Yield3-151 (pmol) . | Location3-152 . | . | ||
| . | . | . | . | . | . | . |
| Tubulin β chain | TSRGSQQYRALTVPELT | 180 | C | Widespread | ||
| HLA-DQ(5) β chain | NSQKEVLEGTRAELDTV | 67 | M | Widespread | ||
| Integrin β 5 subunit | DAPEGGFDAVLQAAVXK | 27 | M | Widespread | ||
| Erythrocyte membrane protein p55 | VPYTTRPPRKSEEDGKEY | 32 | M | Widespread (erythroid) | ||
| Prolidase | AVDDVQYVDEIASVLTSQ | 21 | L | Widespread (secreted) | ||
| Thrombospondin 1 | DNXPFHYNPAQYDYD | 45 | L | Widespread (secreted) | ||
| MRP14 | EGPGHHHKPGLGEG | 45 | M/C | Myeloid | ||
| Leukotriene-B4 Omega-Hydroxylase | WPDPEVYDPFRFDPKNIK | 89 | M | Myeloid | ||
| Granzyme H | QPFLLLLAFLLTP | 93 | L | T lymphoid, NK cells | ||
| Precursor Protein . | Sequence/Anchors3-150 . | Predominant Distribution . | ||||
|---|---|---|---|---|---|---|
| . | i+3+8 . | Yield3-151 (pmol) . | Location3-152 . | . | ||
| . | . | . | . | . | . | . |
| Tubulin β chain | TSRGSQQYRALTVPELT | 180 | C | Widespread | ||
| HLA-DQ(5) β chain | NSQKEVLEGTRAELDTV | 67 | M | Widespread | ||
| Integrin β 5 subunit | DAPEGGFDAVLQAAVXK | 27 | M | Widespread | ||
| Erythrocyte membrane protein p55 | VPYTTRPPRKSEEDGKEY | 32 | M | Widespread (erythroid) | ||
| Prolidase | AVDDVQYVDEIASVLTSQ | 21 | L | Widespread (secreted) | ||
| Thrombospondin 1 | DNXPFHYNPAQYDYD | 45 | L | Widespread (secreted) | ||
| MRP14 | EGPGHHHKPGLGEG | 45 | M/C | Myeloid | ||
| Leukotriene-B4 Omega-Hydroxylase | WPDPEVYDPFRFDPKNIK | 89 | M | Myeloid | ||
| Granzyme H | QPFLLLLAFLLTP | 93 | L | T lymphoid, NK cells | ||
Alignment of self-peptide sequences along the motifs of HLA-DR1 and DR3 according to the notation introduced by Hill et al.20 The italicized letter i indicates the first or N terminal anchor residue, followed by the number of intervening amino acid residues to the last or C terminal anchor position (DR 1 = i + 8, DR 3 = i + 3).19
The total yield of peptides in Table 3 accounted for approximately 27% of the mass of material found in all the HLA-DR fractions combined.
C, cytoplasmic; L, lumenal; M, membrane associated.