Table 2. HLA-B63, B5101–Bound Peptides... | American Society of Hematology

Table 2.

HLA-B63, B5101–Bound Peptides From CD34⁺ Blast Cells of a Patient With CML

Precursor Protein	Sequence^*										Yield^† (pmol)	Location^‡	Predominant Distribution
	1	2	3	4	5	6	7	8	9	10
c-cbl	I	L	R	E	F	V	S	I	S		5	C	Widespread
G 3-PDH	M	A	H	M	A	S	K	E	V		81	C	Widespread
Transketolase	I	D	R	D	A	I	A	Q	A	V	17	C	Widespread
AF6	S	L	N	D	A	P	F	S	P		9	C	Widespread (?)
a-Myb	I	M	T	E	Q	A	R	R	Y		18	C	Widespread
Rhombotin 1	M	V	L	D	K	E	D	G	V	P	21	C	Widespread (?)
P19-INK4D	V	E	H	G	A	D	V	N	V		14	C	Widespread
UBR1 (yeast)	X	P	A	Y	H	D	H	S	P		15	C	Widespread
Flavin monooxygenase	F	P	F	P	E	D	Y	P	N		15	M	Widespread
CD44	V	T	V	G	D	S	N	S	N	V	32	M	Widespread
CD5	A	E	N	P	T	A	S	H	V	D	8	M	Lymphoid (myeloid)
Proteinase 3	R	L	V	N	V	V	L	G	A	H	33	L	Myeloid
Tryptase	L	A	S	R	A	Y	A	A	P		25	L	Myeloid

Precursor Protein	Sequence^*										Yield^† (pmol)	Location^‡	Predominant Distribution
	1	2	3	4	5	6	7	8	9	10
c-cbl	I	L	R	E	F	V	S	I	S		5	C	Widespread
G 3-PDH	M	A	H	M	A	S	K	E	V		81	C	Widespread
Transketolase	I	D	R	D	A	I	A	Q	A	V	17	C	Widespread
AF6	S	L	N	D	A	P	F	S	P		9	C	Widespread (?)
a-Myb	I	M	T	E	Q	A	R	R	Y		18	C	Widespread
Rhombotin 1	M	V	L	D	K	E	D	G	V	P	21	C	Widespread (?)
P19-INK4D	V	E	H	G	A	D	V	N	V		14	C	Widespread
UBR1 (yeast)	X	P	A	Y	H	D	H	S	P		15	C	Widespread
Flavin monooxygenase	F	P	F	P	E	D	Y	P	N		15	M	Widespread
CD44	V	T	V	G	D	S	N	S	N	V	32	M	Widespread
CD5	A	E	N	P	T	A	S	H	V	D	8	M	Lymphoid (myeloid)
Proteinase 3	R	L	V	N	V	V	L	G	A	H	33	L	Myeloid
Tryptase	L	A	S	R	A	Y	A	A	P		25	L	Myeloid

Standard IUPAC amino acid abbreviations are used. X denotes an unidentifiable PTH-amino acid.

†

Initial yield of peptides calculated from the repetitive yields of PTH amino acid derivatives of the sequence. The total yield of peptides in Table 2 accounted for approximately 1.2% of the mass of material found in all the HLA-B fractions combined.

‡

C, cytoplasmic; L, lumenal; M, membrane associated.

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