E21R and MoAb cA2 Prevent Engraftment of JMML, But Not of Normal Bone Marrow Cells in SCID/NOD Mice
| Intervention . | Donor . | Cell Content in Mice Femoral Marrow (%) . | ||
|---|---|---|---|---|
| . | . | Human XY . | Human XX . | Mouse . |
| Untreated | Normal female | 0; 0; 0 | 56; 48; 51 | 44; 52; 49 |
| Untreated | JMML male | 78; 84; 80 | 0; 0; 0 | 21; 16; 20 |
| Untreated | Normal female + JMML male | 54; 49; 58 | 41; 39; 30 | 5; 12; 12 |
| E21R + cA2 | Normal female | 0; 0; 0 | 47; 63; 56 | 53; 37; 44 |
| E21R + cA2 | JMML male | 0; 0; 0 | 0; 0; 0 | 100; 100; 100 |
| E21R + cA2 | Normal female + JMML male | 0; 0; 0 | 53; 36; 47 | 47; 64; 53 |
| Intervention . | Donor . | Cell Content in Mice Femoral Marrow (%) . | ||
|---|---|---|---|---|
| . | . | Human XY . | Human XX . | Mouse . |
| Untreated | Normal female | 0; 0; 0 | 56; 48; 51 | 44; 52; 49 |
| Untreated | JMML male | 78; 84; 80 | 0; 0; 0 | 21; 16; 20 |
| Untreated | Normal female + JMML male | 54; 49; 58 | 41; 39; 30 | 5; 12; 12 |
| E21R + cA2 | Normal female | 0; 0; 0 | 47; 63; 56 | 53; 37; 44 |
| E21R + cA2 | JMML male | 0; 0; 0 | 0; 0; 0 | 100; 100; 100 |
| E21R + cA2 | Normal female + JMML male | 0; 0; 0 | 53; 36; 47 | 47; 64; 53 |
Mice were injected with bone marrow cells from either a healthy female donor, or from a male JMML patient, or with an equal mixture of normal and JMML cells. The mice were either left untreated or given a combined treatment with E21R and MoAb cA2 for 4 weeks from the time of transplantation. The fractions of human XY, XX, and mouse cells collected from mouse femoral marrow were quantitated using a fluorescent in situ hybridization. The values represent the percentage of 300 counted bone marrow cells. Data from three different mice are shown.