Hematologic Parameters of Mice in These Studies
| A. . | |||
|---|---|---|---|
| Parameters . | Transfusion Treatment Given to Jaundiced Mice . | ||
| . | PBC >4 wks* . | PBC + BM† . | PBC 1ׇ . |
| Hematocrit (%) | 18.9 ± 1.3 (4)ρ1-155 | 19.3 ± 3.4 (3) | 22.8 ± 3.6 (15)ρ |
| RBC (×1012/L) | 4.00 ± 0.30 (4) | 3.60 ± 0.20 (3) | 4.40 ± 0.80 (15) |
| MCV ( μm3) | 47.8 ± 3.6 (4) | 53.4 ± 7.0 (3) | 52.6 ± 4.6 (15) |
| Hemoglobin (g/dL) | 3.8 ± 0.6 (4) | 3.4 ± 0.5 (3) | 4.2 ± 0.7 (15) |
| MCHC (%) | 20.1 ± 2.0 (4) | 17.8 ± 1.8 (3) | 18.3 ± 1.5 (15) |
| A. . | |||
|---|---|---|---|
| Parameters . | Transfusion Treatment Given to Jaundiced Mice . | ||
| . | PBC >4 wks* . | PBC + BM† . | PBC 1ׇ . |
| Hematocrit (%) | 18.9 ± 1.3 (4)ρ1-155 | 19.3 ± 3.4 (3) | 22.8 ± 3.6 (15)ρ |
| RBC (×1012/L) | 4.00 ± 0.30 (4) | 3.60 ± 0.20 (3) | 4.40 ± 0.80 (15) |
| MCV ( μm3) | 47.8 ± 3.6 (4) | 53.4 ± 7.0 (3) | 52.6 ± 4.6 (15) |
| Hemoglobin (g/dL) | 3.8 ± 0.6 (4) | 3.4 ± 0.5 (3) | 4.2 ± 0.7 (15) |
| MCHC (%) | 20.1 ± 2.0 (4) | 17.8 ± 1.8 (3) | 18.3 ± 1.5 (15) |
| B. . | ||||
|---|---|---|---|---|
| Parameters . | Genotype . | |||
| . | +/+ . | ja/+ . | ja/ja . | sph/sph . |
| Hematocrit (%) | 45.0 ± 4.0 (6)1-155 | 45.4 ± 4.4 (17) | 21.6 ± 3.6 (22)1-154 | 22.5 ± 5.5 (6)1-167 |
| RBC (×1012/L) | 9.15 ± 1.73 (6) | 10.60 ± 1.20 (16) | 4.20 ± 0.70 (22)1-154 | 3.74 ± 0.50 (6)1-167 |
| MCV ( μm3) | 50.0 ± 7.3 (6) | 42.7 ± 2.3 (16)1-167 | 51.8 ± 4.9 (22) | 59.5 ± 7.8 (6) |
| Hemoglobin (g/dL) | 13.5 ± 1.9 (6) | 14.1 ± 1.4 (17) | 4.0 ± 0.7 (22)1-154 | 3.7 ± 0.7 (6)1-167 |
| MCHC (%) | 29.8 ± 2.0 (6) | 31.0 ± 1.4 (17) | 18.6 ± 1.7 (22)1-154 | 16.9 ± 1.5 (6)1-167 |
| B. . | ||||
|---|---|---|---|---|
| Parameters . | Genotype . | |||
| . | +/+ . | ja/+ . | ja/ja . | sph/sph . |
| Hematocrit (%) | 45.0 ± 4.0 (6)1-155 | 45.4 ± 4.4 (17) | 21.6 ± 3.6 (22)1-154 | 22.5 ± 5.5 (6)1-167 |
| RBC (×1012/L) | 9.15 ± 1.73 (6) | 10.60 ± 1.20 (16) | 4.20 ± 0.70 (22)1-154 | 3.74 ± 0.50 (6)1-167 |
| MCV ( μm3) | 50.0 ± 7.3 (6) | 42.7 ± 2.3 (16)1-167 | 51.8 ± 4.9 (22) | 59.5 ± 7.8 (6) |
| Hemoglobin (g/dL) | 13.5 ± 1.9 (6) | 14.1 ± 1.4 (17) | 4.0 ± 0.7 (22)1-154 | 3.7 ± 0.7 (6)1-167 |
| MCHC (%) | 29.8 ± 2.0 (6) | 31.0 ± 1.4 (17) | 18.6 ± 1.7 (22)1-154 | 16.9 ± 1.5 (6)1-167 |
Age range of jaundiced and control mice studied was 2 to 9.5 months old, average 4.5 months old, one jaundiced mouse moribund at time of analysis. Age range of spherocytic mice studied was 2.75 to 4.25 months old, average 3.8 months old, none moribund at time of analysis.
Abbreviations: PBC, peripheral blood cell; BM, bone marrow; RBC, red blood cell count; MCHC, mean corpuscular hemoglobin concentration; MCV, mean cell volume.
Jaundiced mice given weekly PBC transfusions for greater than 4 weeks.
Jaundiced mice given a PBC transfusion in addition to bone marrow cells at days 1, 2, and 3 after birth; a few mice were given one additional PBC transfusion a few days later.
Jaundiced mice given a single PBC transfusion at days 1, 2, or 3 after birth.
ρ Significant difference (P < .05).
Mean ± standard deviation; number of observations in parentheses.
Extremely significant difference (P < .0005).
Very significant difference (P < .005).
For part A, significance noted is in hematocrit values between TF 1× and TF >4 weeks. For part B, significance when compared with +/+ values.