Inducibility of E- and P-Selectin on Primary MBEs Is Reduced by ACM
| Treatment . | E-Selectin . | E-Selectin + ACM . | P-Selectin . | P-Selectin + ACM . |
|---|---|---|---|---|
| LPS (n = 7) | ||||
| (10 μg/mL, 3 h) | + | ⊘ to +/− | +/− | ⊘ |
| IL-1 (n = 3) | ||||
| (5 ng/mL, 3 h) | ++ | + | + | +/− |
| TNF-α (n = 3) | ||||
| (5 nmol/L, 3 h) | + | +/− | +/− | +/− to ⊘ |
| Treatment . | E-Selectin . | E-Selectin + ACM . | P-Selectin . | P-Selectin + ACM . |
|---|---|---|---|---|
| LPS (n = 7) | ||||
| (10 μg/mL, 3 h) | + | ⊘ to +/− | +/− | ⊘ |
| IL-1 (n = 3) | ||||
| (5 ng/mL, 3 h) | ++ | + | + | +/− |
| TNF-α (n = 3) | ||||
| (5 nmol/L, 3 h) | + | +/− | +/− | +/− to ⊘ |
Semiquantitative analysis of surface immunofluorescence staining on vWF-positive MBEs. Staining for PECAM-1 as a positive control was considered +++ (see Fig 9): +++, strongly positive staining; ++, positive staining; +, low staining intensity; +/−, very low staining intensity; ⊘, no staining.