Transduction Efficiency of LTC-IC by the LNL6 or 9456 Ribozyme Retroviral Vectors
| Experiment No. . | G418-Resistant CFU* . | |
|---|---|---|
| . | LNL6 . | p9456t . |
| 1 | 35% | 37% |
| 2 | 54% | 56% |
| 3 | 33% | 30% |
| Experiment No. . | G418-Resistant CFU* . | |
|---|---|---|
| . | LNL6 . | p9456t . |
| 1 | 35% | 37% |
| 2 | 54% | 56% |
| 3 | 33% | 30% |
Transduced CD34+ cells were cultured on irradiated bone marrow stroma for 5 weeks and then cultured in methylcellulose in the presence and absence of G418. Colony counts reflect the sum of both CFU-GM and BFU-E.