Dose-Dependent Induction by Bryo-1 of Cytokine Secretion From Human Monocytes
| Treatment . | Cytokines (pg/mL) . | |||
|---|---|---|---|---|
| . | IL-1β . | IL-8 . | TNF-α . | IL-6 . |
| Donor 1 | ||||
| Medium | 0 | 399 | 0 | 48 |
| Bryo 0.001 ng/mL | 117 | 1,514 | 77 | 2,925 |
| Bryo 0.01 ng/mL | 146 | 2,914 | 189 | 4,831 |
| Bryo 0.1 ng/mL | 89,400 | 16,413 | 11,700 | 6,051 |
| Donor 2 | ||||
| Medium | 0 | 1,880 | 0 | 0 |
| Bryo 0.001 ng/mL | 12 | 3,272 | 33 | 45 |
| Bryo 0.01 ng/mL | 41 | 7,483 | 62 | 191 |
| Bryo 0.1 ng/mL | 400 | 84,260 | 1,000 | 2,438 |
| Treatment . | Cytokines (pg/mL) . | |||
|---|---|---|---|---|
| . | IL-1β . | IL-8 . | TNF-α . | IL-6 . |
| Donor 1 | ||||
| Medium | 0 | 399 | 0 | 48 |
| Bryo 0.001 ng/mL | 117 | 1,514 | 77 | 2,925 |
| Bryo 0.01 ng/mL | 146 | 2,914 | 189 | 4,831 |
| Bryo 0.1 ng/mL | 89,400 | 16,413 | 11,700 | 6,051 |
| Donor 2 | ||||
| Medium | 0 | 1,880 | 0 | 0 |
| Bryo 0.001 ng/mL | 12 | 3,272 | 33 | 45 |
| Bryo 0.01 ng/mL | 41 | 7,483 | 62 | 191 |
| Bryo 0.1 ng/mL | 400 | 84,260 | 1,000 | 2,438 |
Monocytes were cultured at 2 × 106/mL in medium alone or supplemented with increasing concentrations of bryo-1. Supernatants were collected after 18 hours and assayed for the indicated cytokines by specific ELISA. Bryo-1 conversion factor: 1.0 ng/mL = 0.904 nmol/L.